Huang Shuting, Huang Xia, Li Shuang, Zhu Mingjun, Zhuo Min
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.
PeerJ. 2019 Nov 4;7:e7941. doi: 10.7717/peerj.7941. eCollection 2019.
Cynomolgus macaques (, ) have been used as important experimental animal models for carrying out biomedical researches. The results of biomedical experiments strongly depend on the immunogenetic background of animals, especially on the diversity of major histocompatibility complex (MHC) alleles. However, there is much less information available on the polymorphism of MHC class I genes in cynomolgus macaques, than is currently available for humans. In this study, we have identified 40 and 60 exons 2 and 3 sequences from 30 unrelated cynomolgus macaques of Vietnamese origin. Among these alleles, 28 are novel. As for the remaining 72 known alleles, 15 alleles are shared with other cynomolgus macaque populations and 32 are identical to alleles previously reported in other macaque species. A potential recombination event was observed between and . In addition, the genes were found to be more diverse than human and the functional residues for peptide binding sites (PBS) or TCR binding sites (TBS) in have greater variability than that for non-PBS or non-TBS regions. Overall, this study provides important information on the diversity of and alleles from Vietnamese origin, which may help researchers to choose the most appropriate animals for their studies.
食蟹猴（ ， ）已被用作开展生物医学研究的重要实验动物模型。生物医学实验的结果在很大程度上取决于动物的免疫遗传背景，尤其是主要组织相容性复合体（MHC）等位基因的多样性。然而，与目前人类的情况相比，关于食蟹猴MHC I类基因多态性的信息要少得多。在本研究中，我们从30只越南原产的无亲缘关系的食蟹猴中鉴定出了40个和60个外显子2和3序列。在这些等位基因中，有28个是新的。至于其余72个已知等位基因，有15个等位基因与其他食蟹猴种群共享且32个与先前在其他猕猴物种中报道的等位基因相同。在 和 之间观察到一个潜在的重组事件。此外，发现 基因比人类 基因具有更多样性，并且 中肽结合位点（PBS）或TCR结合位点（TBS）的功能残基比非PBS或非TBS区域具有更大的变异性。总体而言，本研究提供了关于越南原产 等位基因多样性的重要信息，这可能有助于研究人员为其研究选择最合适的动物。
