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梅毒螺旋体的34千道尔顿膜免疫原是一种脂蛋白。

The 34-kilodalton membrane immunogen of Treponema pallidum is a lipoprotein.

作者信息

Swancutt M A, Radolf J D, Norgard M V

机构信息

Department of Microbiology, University of Texas, Southwestern Medical Center, Dallas 75235.

出版信息

Infect Immun. 1990 Feb;58(2):384-92. doi: 10.1128/iai.58.2.384-392.1990.

DOI:10.1128/iai.58.2.384-392.1990
PMID:2404869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC258467/
Abstract

Treponema pallidum subsp. pallidum and Escherichia coli incorporated exogenous [3H]palmitate into the 34-kilodalton (kDa) pathogen-specific antigen of T. pallidum. Radiolabeled fatty acid remained associated with the protein upon immunoprecipitation and after boiling in sodium dodecyl sulfate, acetone precipitation, and extensive extractions in organic solvents, suggesting that the fatty acid was covalently bound to the protein. Detection of [3H]palmitate after alkaline and acid hydrolyses confirmed the identity of the incorporated label. Globomycin inhibited maturation of the recombinant 34-kDa antigen, suggesting that E. coli uses the lipoprotein-specific signal peptidase II to process the treponemal antigen. Globomycin also inhibited processing of the 34-kDa antigen, as well as the 44.5- and 15-kDa antigens, in T. pallidum, implying that T. pallidum also possesses the lipoprotein export pathway common to both gram-negative and gram-positive bacteria. Ethanol inhibited processing of the 34-kDa antigen in minicells, suggesting that the 34-kDa antigen normally is translocated through the cytoplasmic membrane. Comparison of the Triton X-114 phase partitioning behavior of the 34-kDa antigen produced either by minicells or by a cell-free translation system indicated that the covalent attachment of fatty acid conferred hydrophobic biochemical properties to the 34-kDa antigen, consistent with the hypothesis that the attached lipid anchors the 34-kDa antigen into the membrane.

摘要

梅毒螺旋体苍白亚种和大肠杆菌将外源性[3H]棕榈酸掺入梅毒螺旋体34千道尔顿（kDa）的病原体特异性抗原中。放射性标记的脂肪酸在免疫沉淀后以及在十二烷基硫酸钠中煮沸、丙酮沉淀和在有机溶剂中大量萃取后仍与蛋白质结合，这表明脂肪酸与蛋白质共价结合。碱水解和酸水解后对[3H]棕榈酸的检测证实了掺入标记的身份。球蛋白霉素抑制重组34-kDa抗原的成熟，这表明大肠杆菌利用脂蛋白特异性信号肽酶II来加工梅毒螺旋体抗原。球蛋白霉素还抑制梅毒螺旋体中34-kDa抗原以及44.5-kDa和15-kDa抗原的加工，这意味着梅毒螺旋体也拥有革兰氏阴性菌和革兰氏阳性菌共有的脂蛋白输出途径。乙醇抑制小细胞中34-kDa抗原的加工，这表明34-kDa抗原通常通过细胞质膜转运。对小细胞或无细胞翻译系统产生的34-kDa抗原的Triton X-114相分配行为的比较表明，脂肪酸的共价连接赋予34-kDa抗原疏水的生化特性，这与附着的脂质将34-kDa抗原锚定在膜中的假设一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/9377e776c5aa/iai00050-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/0daf789a8fac/iai00050-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/be95cb2d0275/iai00050-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/43c45f1f5fd9/iai00050-0117-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/4246de00d5c3/iai00050-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/b5363d235cac/iai00050-0118-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/86f866454750/iai00050-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/9377e776c5aa/iai00050-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/0daf789a8fac/iai00050-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/be95cb2d0275/iai00050-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/43c45f1f5fd9/iai00050-0117-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/4246de00d5c3/iai00050-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/b5363d235cac/iai00050-0118-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/86f866454750/iai00050-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/606b/258467/9377e776c5aa/iai00050-0120-a.jpg

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