Smoczer Cristine, Hooker Lara, Sachani Saqib S, Crawford Michael J
Biological Sciences, University of Windsor, Windsor, ON, Canada.
Methods Mol Biol. 2014;1082:143-54. doi: 10.1007/978-1-62703-655-9_10.
Microinjection has a long and distinguished history in Xenopus and has been used to introduce a surprisingly diverse array of agents into embryos by both intra- and intercellular means. In addition to nuclei, investigators have variously injected peptides, antibodies, biologically active chemicals, lineage markers, mRNA, DNA, morpholinos, and enzymes. While enumerating many of the different microinjection approaches that can be taken, we will focus upon the mechanical operations and options available to introduce mRNA, DNA, and morpholinos intracellularly into early stage embryos for the study of neurogenesis.
显微注射在非洲爪蟾中有着悠久而卓越的历史，并且已被用于通过细胞内和细胞间的方式将一系列惊人多样的物质引入胚胎。除了细胞核，研究人员还分别注射了肽、抗体、生物活性化学物质、谱系标记物、信使核糖核酸（mRNA）、脱氧核糖核酸（DNA）、吗啉代寡核苷酸和酶。在列举可以采用的许多不同显微注射方法时，我们将重点关注将信使核糖核酸、脱氧核糖核酸和吗啉代寡核苷酸细胞内导入早期胚胎以研究神经发生的机械操作和可用选项。
