Department of Histology and Embryology, Medical University of Lublin, Poland.
Arch Med Sci. 2013 Aug 30;9(4):719-23. doi: 10.5114/aoms.2013.37273. Epub 2013 Aug 29.
The aim of the present study was to investigate expression of HSP70 and p-53 proteins as mechanisms of protection of the renal tubular epithelial cells from l-arginine that induces cellular stress.
The study material consisted of 16 white Wistar female rats. The rats were divided into 2 equal groups. The rats in the experimental group received L-arginine 40 mg/kg body weight per capita every other day for 2 weeks and were decapitated after 3 weeks of the experiment. After decapitation, specimens from the kidney were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Proteins HSP70 and p-53 on slides were detected using the standard three-step immunohistochemical method.
The quantitative evaluation of HSP70 and p-53 expression showed that the area occupied with positive HSP70 and p-53 reaction in the rat renal tubular cells of the experimental group (p-53: 2835.44 ±254.72 µm(2); HSP70: 24111.42 ±4290.88 µm(2)) was more statistically significant than the control group (p-53: 1882.05 ±466.43 µm(2); HSP70: 11388.63 ±1455.24 µm(2)). In the present study, the dose of L-arginine was similar to the one that was used in the gestosis treatment of pregnant women.
The renal epithelial cells responded to L-arginine therapy, increasing expression of HSP70 and p-53 proteins. The study showed that L-arginine as a donor of exogenous nitric oxide has a disruptive effect on the renal tubular cells of rat kidneys. Thus it is going to be a subject of the author's future investigations.
本研究的目的是研究 HSP70 和 p-53 蛋白的表达作为诱导细胞应激的 l-精氨酸保护肾小管上皮细胞的机制。
研究材料包括 16 只白色 Wistar 雌性大鼠。将大鼠分为两组。实验组大鼠每两天接受 40mg/kg 体重的 L-精氨酸，实验 3 周后断头。断头后，从肾脏采集标本，用 10%福尔马林固定，然后嵌入石蜡块。使用标准三步免疫组织化学法检测载玻片上的 HSP70 和 p-53 蛋白。
HSP70 和 p-53 表达的定量评估表明，实验组大鼠肾小管细胞中 HSP70 和 p-53 阳性反应面积（p-53：2835.44±254.72µm(2)；HSP70：24111.42±4290.88µm(2)）明显大于对照组（p-53：1882.05±466.43µm(2)；HSP70：11388.63±1455.24µm(2)）。在本研究中，L-精氨酸的剂量与用于治疗孕妇先兆子痫的剂量相似。
肾上皮细胞对 L-精氨酸治疗作出反应，增加 HSP70 和 p-53 蛋白的表达。研究表明，作为外源性一氧化氮供体的 L-精氨酸对大鼠肾脏肾小管细胞具有破坏作用。因此，这将是作者未来研究的课题。
