Department of Pneumology and Allergy, Medical University of Lodz, Poland.
Arch Med Sci. 2012 May 9;8(2):277-85. doi: 10.5114/aoms.2012.28555.
Eicosanoids and oxidants play an important role in inflammation, but their role in chronic obstructive pulmonary disease (COPD) is uncertain. In this study we hypothesized that levels of exhaled leukotrienes, prostaglandins and biomarkers of oxidative stress are increased in infectious exacerbations of COPD and that they decrease after antibiotic therapy.
Cysteinyl-leukotrienes (LTs), leukotriene B(4) (LTB(4)), prostaglandin E(4), hydrogen peroxide (H(2)O(2)) and 8-isoprostane were measured in exhaled breath condensate (EBC) in 16 COPD patients with infectious exacerbations (mean age 64 ±12 years, 13 male) on day 1, during antibiotic therapy (days 2-4), 2-4 days after therapy and at a follow-up visit when stable (21-28 days after therapy).
There was a significant fall in concentration of cys-LTs, LTB(4) and 8-isoprostane at visit 3 compared to day 1 (cys-LTs: 196.5 ±38.4 pg/ml vs. 50.1 ±8.2 pg/ml, p < 0.002; LTB(4): 153.6 ±25.5 pg/ml vs. 71.9 ±11.3 pg/ml, p < 0.05; 8-isoprostane: 121.4 ±14.6 pg/ml vs. 56.1 ±5.2 pg/ml, p < 0.03, respectively). Exhaled H(2)O(2) was higher on day 1 compared to that at visits 2 and 3 (0.74 ±0.046 µM vs. 0.52 ±0.028 µM and 0.35 ±0.029 µM, p < 0.01 and p < 0.01, respectively). Exhaled PGE(2) levels did not change during exacerbations of COPD. Exhaled eicosanoids and H(2)O(2) in EBC measured at the follow-up visit (stable COPD) were significantly higher compared to those from healthy subjects.
We conclude that eicosanoids and oxidants are increased in infectious exacerbations of COPD. They are also elevated in the airways of stable COPD patients compared to healthy subjects.
类二十烷酸和氧化剂在炎症中起着重要作用，但它们在慢性阻塞性肺疾病（COPD）中的作用尚不确定。在这项研究中，我们假设在 COPD 的感染性加重期，呼出的白三烯、前列腺素和氧化应激生物标志物的水平升高，并且在抗生素治疗后会降低。
在 16 例患有感染性加重的 COPD 患者（平均年龄 64±12 岁，13 名男性）中，于第 1 天、抗生素治疗期间（第 2-4 天）、治疗后 2-4 天和稳定时（治疗后 21-28 天）测量呼气冷凝物（EBC）中的半胱氨酸白三烯（LTs）、白细胞三烯 B4（LTB4）、前列腺素 E4、过氧化氢（H2O2）和 8-异前列腺素。
与第 1 天相比，第 3 次就诊时 cys-LTs、LTB4 和 8-异前列腺素的浓度明显下降（cys-LTs：196.5±38.4 pg/ml 与 50.1±8.2 pg/ml，p<0.002；LTB4：153.6±25.5 pg/ml 与 71.9±11.3 pg/ml，p<0.05；8-异前列腺素：121.4±14.6 pg/ml 与 56.1±5.2 pg/ml，p<0.03，分别）。与第 2 次和第 3 次就诊相比，第 1 天呼气 H2O2 水平更高（0.74±0.046 µM 与 0.52±0.028 µM 和 0.35±0.029 µM，p<0.01 和 p<0.01，分别）。COPD 加重期间，EBC 中呼出的 PGE2 水平没有变化。在稳定的 COPD 患者中，随访时（稳定的 COPD）测量的呼出的类二十烷酸和 H2O2 明显高于健康受试者。
我们得出结论，在 COPD 的感染性加重期，类二十烷酸和氧化剂增加。与健康受试者相比，它们在稳定的 COPD 患者的气道中也升高。
