Furtado João M, Davies Michael H, Choi Dongseok, Lauer Andreas K, Appukuttan Binoy, Bailey Steven T, Rahman Hassan T, Payne John F, Stempel Andrew J, Mohs Kathleen, Powers Michael R, Yeh Steven, Smith Justine R
Casey Eye Institute, Oregon Health & Science University, Portland, OR.
Transl Vis Sci Technol. 2012 Sep 18;1(2):5. doi: 10.1167/tvst.1.2.5. eCollection 2012.
Oxygen-induced retinopathy in the mouse is the standard experimental model of retinopathy of prematurity. Assessment of the pathology involves in vitro analysis of retinal vaso-obliteration and retinal neovascularization. The authors studied the clinical features of oxygen-induced retinopathy in vivo using topical endoscopy fundus imaging (TEFI), in comparison to standard investigations, and evaluated a system for grading these features.
Postnatal day (P)7 mice were exposed to 75% oxygen for five days to induce retinopathy or maintained in room air as controls. Retinal vascular competence was graded against standard photographs by three masked graders. Retinal photographs were obtained at predetermined ages using TEFI. Postmortem, retinal vaso-obliteration was measured in whole mounts with labeled vasculature, and retinal neovascularization was quantified in hematoxylin- and eosin-stained ocular cross sections.
Fundus photography by TEFI was possible from P15, when retinal vascular incompetence, including dilatation and tortuosity, was significant in mice with oxygen-induced retinopathy in comparison to controls. Vascular incompetence peaked in severity at P17 and persisted through P25. Comparison with in vitro analyses indicated that vascular changes were most severe after retinal avascularity had begun to decrease in area, and coincident with the maximum of retinal neovascularization. A weighted Fleiss-Cohen kappa indicated good intra- and interobserver agreement for a 5-point grading system.
Topical endoscopy fundus imaging demonstrates retinal vascular incompetence in mice with oxygen-induced retinopathy. The technique complements standard postmortem analysis for following the course of the model.
Topical endoscopy fundus imaging has application in the evaluation of novel biologic drugs for retinopathy of prematurity.
小鼠氧诱导性视网膜病变是早产儿视网膜病变的标准实验模型。病理学评估涉及对视网膜血管闭塞和视网膜新生血管形成的体外分析。作者使用局部内镜眼底成像（TEFI）在体内研究了氧诱导性视网膜病变的临床特征，并与标准检查进行了比较，还评估了一种对这些特征进行分级的系统。
将出生后第7天（P7）的小鼠暴露于75%氧气中5天以诱导视网膜病变，或置于室内空气中作为对照。由三名不知情的评分者根据标准照片对视网膜血管功能进行分级。使用TEFI在预定年龄获取视网膜照片。死后，在带有标记血管的整装标本中测量视网膜血管闭塞情况，并在苏木精和伊红染色的眼部横断面中对视网膜新生血管形成进行定量分析。
从P15开始就可以通过TEFI进行眼底摄影，此时与对照组相比，氧诱导性视网膜病变小鼠的视网膜血管功能不全，包括扩张和迂曲，较为明显。血管功能不全在P17时严重程度达到峰值，并持续到P25。与体外分析相比表明，在视网膜无血管区面积开始减少后，血管变化最为严重，且与视网膜新生血管形成的最大值同时出现。加权Fleiss-Cohen卡方检验表明，对于一个5分分级系统，观察者内和观察者间的一致性良好。
局部内镜眼底成像显示了氧诱导性视网膜病变小鼠的视网膜血管功能不全。该技术补充了标准的死后分析，用于跟踪模型的病程。
局部内镜眼底成像在评估用于早产儿视网膜病变的新型生物药物方面具有应用价值。
