Griffith R W, Kramer T T, McRill C
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011 USA.
Am J Vet Res. 1984 Jul;45(7):1336-41.
The effects of the immunosuppressive agent cyclophosphamide (CY) on the immune response of pigs given IM challenge inoculations of a moderately virulent strain of Salmonella cholerae-suis var kunzendorf were examined. Five groups of Yorkshire-cross pigs (approx 6 kg) were given Salmonella, CY, or both at various times after the 1st of a series of doses of CY was given. The drug was administered subcutaneously in 3 doses, 2 days between doses, at a rate of 20 mg/kg of body weight. Cyclophosphamide was observed to produce significant numerical decrease of circulating leukocytes, especially the polymorphonuclear neutrophils. Circulating lymphocyte numbers were reduced to 40% to 60% of base-line values. Pigs (group 4) given 3 x 10(6) S cholerae-suis IM at the time the initial CY dose was given were clinically ill during days 6 to 12; 2 of the 5 pigs died. In contrast, pigs (group 5) given 3 x 10(6) S cholerae-suis at the time of the 3rd dosing with CY did not become clinically ill until 10 days later. A significant increase in antibody titer to S cholerae-suis was delayed in this latter group beyond that of the groups of pigs (group 4) inoculated on the 1st dosing with CY and of pigs (group 6) not given CY. A significant and prolonged increase in mean rectal temperature was observed in those pigs challenge inoculated at the time of the initial CY dose. Pigs also were sensitized to Mycobacterium avium 2 weeks before CY administration. Delayed hypersensitivity reactions were depressed in pigs treated with CY at the time of testing.
研究了免疫抑制剂环磷酰胺（CY）对经肌内接种中等毒力的猪霍乱沙门氏菌昆赞多夫菌株进行激发接种的猪免疫反应的影响。将五组约克夏杂交猪（约6千克）在给予一系列剂量的CY中的第一剂后的不同时间给予沙门氏菌、CY或两者。该药物以20毫克/千克体重的剂量皮下注射3次，每次给药间隔2天。观察到环磷酰胺使循环白细胞数量显著减少，尤其是多形核中性粒细胞。循环淋巴细胞数量降至基线值的40%至60%。在给予初始CY剂量时经肌内接种3×10⁶猪霍乱沙门氏菌的猪（第4组）在第6至12天出现临床疾病；5头猪中有2头死亡。相比之下，在第三次给予CY时接种3×10⁶猪霍乱沙门氏菌的猪（第5组）直到10天后才出现临床疾病。后一组猪对猪霍乱沙门氏菌的抗体滴度显著升高的时间比在第一次给予CY时接种的猪组（第4组）和未给予CY的猪组（第6组）延迟。在给予初始CY剂量时进行激发接种的那些猪中观察到平均直肠温度显著且持续升高。在给予CY前2周，猪也对鸟分枝杆菌致敏。在测试时用CY治疗的猪中，迟发型超敏反应受到抑制。
