Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University , Guangzhou 510642, PR China.
J Agric Food Chem. 2013 Oct 2;61(39):9510-5. doi: 10.1021/jf4028602. Epub 2013 Sep 19.
Tissue deposition and residue depletion profiles of cyadox (Cyx) and its three major metabolites, including 1,4-bisdesoxycyadox (Cy1), 4-desoxycyadox (Cy2), and quinoxaline-2-carboxylic acid (QCA), in pigs after multiple oral administrations were determined. Thirty-five healthy adult pigs were randomly divided into seven groups and orally treated with Cyx at a dosage of 20 mg/kg of body weight for five consecutive days. Each group of five pigs was randomly slaughtered 12, 24, 72, 120, 168, 216, and 264 h after the last dosing, and tissue samples, including muscle, liver, kidney, and fat, were collected and analyzed via the liquid chromatography-tandem mass spectrometry method. The concentration-time data of Cyx and its three metabolites (Cy1, Cy2, and QCA) were analyzed with WinNonlin. Results showed that metabolites of Cyx were quickly generated in swine tissues and the concentrations of QCA in kidney were higher than those of Cyx and other metabolites in all edible tissues. These results provide further insight into the metabolism of Cyx and confirmation of the residue marker and target tissue of Cyx in pigs.
在猪多次口服给药后，测定了噻肟肟（Cyx）及其三种主要代谢物，包括 1,4-双脱氧噻肟肟（Cy1）、4-脱氧噻肟肟（Cy2）和喹喔啉-2-羧酸（QCA）在组织中的沉积和残留消除情况。35 头健康成年猪随机分为 7 组，以 20mg/kg 体重的剂量连续口服 Cyx 5 天。每组 5 头猪在最后一次给药后 12、24、72、120、168、216 和 264 小时随机屠宰，采集肌肉、肝脏、肾脏和脂肪等组织样本，采用液相色谱-串联质谱法进行分析。采用 WinNonlin 对 Cyx 及其三种代谢物（Cy1、Cy2 和 QCA）的浓度-时间数据进行分析。结果表明，Cyx 的代谢物在猪组织中迅速生成，且 QCA 在肾脏中的浓度在所有可食用组织中均高于 Cyx 和其他代谢物。这些结果进一步深入了解了 Cyx 的代谢情况，并确认了 Cyx 在猪中的残留标志物和靶组织。
