转运RNA与核糖体A位点和P位点的结合保护了16S核糖体RNA中两组不同的核苷酸。

Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA.

作者信息

Moazed D, Noller H F

机构信息

Thimann Laboratories, University of California Santa Cruz 95064.

出版信息

J Mol Biol. 1990 Jan 5;211(1):135-45. doi: 10.1016/0022-2836(90)90016-F.

DOI:10.1016/0022-2836(90)90016-F

PMID:2405162

Abstract

Transfer RNA protects a characteristic set of bases in 16 S rRNA from chemical probes when it binds to ribosomes. We used several criteria, based on construction of well-characterized in vitro ribosome-tRNA complexes, to assign these proteins to A or P-site binding. All of these approaches lead to similar conclusions. In the A site, tRNA caused protection of G529, G530, A1492 and A1493 (strongly), and A1408 and G1494 (weakly). In the P site, the protected bases are G693, A794, C795, G926 and G1401 (strong), and A532, G966, G1338 and G1339 (weak). In contrast to what is observed for 23 S rRNA, blocking the release of EF-Tu.GDP from the ribosome by kirromycin has no detectable effect on the protection of bases in 16 S rRNA.

摘要

转运RNA（tRNA）与核糖体结合时，会保护16S核糖体RNA（rRNA）中的一组特定碱基免受化学探针的影响。我们基于构建特征明确的体外核糖体-tRNA复合物，使用了多种标准来将这些蛋白质分配到A或P位点结合。所有这些方法都得出了相似的结论。在A位点，tRNA导致G529、G530、A1492和A1493（强烈）以及A1408和G1494（微弱）受到保护。在P位点，受保护的碱基是G693、A794、C795、G926和G1401（强烈）以及A532、G966、G1338和G1339（微弱）。与23S rRNA的情况相反，奇霉素阻止EF-Tu.GDP从核糖体释放对16S rRNA中碱基的保护没有可检测到的影响。