Huttunen J K, Ehnholm C, Kinnunen P K, Nikkilä E A
Clin Chim Acta. 1975 Sep 16;63(3):335-47. doi: 10.1016/0009-8981(75)90055-8.
A new method for the selective measurement of postheparin plasma lipoprotein lipase and hepatic lipase is described and validated. The activity of lipoprotein lipase is determined at 0.1 M NaCl after removal of hepatic lipase by specific antiserum, and the hepatic lipase is assayed in a medium containing 1.0 M NaCl but no additional serum. The optimal conditions for the determination of the two postheparin plasma triglyceride hydrolases were shown to be similar to those described for the purified enzymes. The new assay methods are simple, accurate and highly specific for the two lipase activities. VLDL and LDL do not interfere with the measurement, making the methods suitable for studies of patients with various hyperlipidemias. More than 90% of the total triglyceride hydrolase activity in postheparin plasma is precipitated with antisera raised against purified human postheparin plasma hepatic lipase and bovine milk lipoprotein lipase. The time and dose dependence of the two postheparin plasma lipase responses differ. For optimal activity of both enzymes, plasma taken 15 minutes after intravenous administration of 100 I.U./kg of heparin, should be used. The activity of postheparin plasma lipoprotein lipase and hepatic lipase in 12 young, healthy males is reported.
本文描述并验证了一种选择性测定肝素后血浆脂蛋白脂肪酶和肝脂肪酶的新方法。通过特异性抗血清去除肝脂肪酶后，在0.1M NaCl条件下测定脂蛋白脂肪酶的活性，而肝脂肪酶则在含有1.0M NaCl但无额外血清的培养基中进行测定。结果表明，测定这两种肝素后血浆甘油三酯水解酶的最佳条件与纯化酶的测定条件相似。新的测定方法简单、准确，对两种脂肪酶活性具有高度特异性。极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）不干扰测定，使得这些方法适用于各种高脂血症患者的研究。用针对纯化的人肝素后血浆肝脂肪酶和牛乳脂蛋白脂肪酶制备的抗血清可沉淀肝素后血浆中超过90%的总甘油三酯水解酶活性。两种肝素后血浆脂肪酶反应的时间和剂量依赖性有所不同。为使两种酶均具有最佳活性，应使用静脉注射100国际单位/千克肝素15分钟后采集的血浆。本文报告了12名年轻健康男性的肝素后血浆脂蛋白脂肪酶和肝脂肪酶活性。
