State Key Lab of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
Talanta. 2013 Oct 15;115:386-93. doi: 10.1016/j.talanta.2013.05.026. Epub 2013 Jun 5.
A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of octachlorostyrene (OCS), a persistent and bioaccumulative toxicant. To achieve the most sensitive antibody, several haptens with different linkers that simulated the special structure of OCS were synthesized and conjugated to carrier proteins. Polyclonal rabbit antibodies against different immunizing antigens were obtained and screened against different coating antigens. Under the optimized conditions, this indirect ELISA shows a linear detection range from 1.4 to 86.3 ng/mL, with an IC50 value of 4.46 ng/mL and a limit of detections (LOD) of 0.1 ng/mL. Twelve kinds of compounds were tested for calculating cross-reactivities, and almost all of them showed little cross-reactivity (<5%). Water and sera samples spiked with OCS were analyzed by ELISA and the achieved recoveries were satisfied with a mean recovery of 92%. This immunoassay can be used as a rapid and convenient tool to monitoring OCS in environmental samples.
建立了一种用于检测八氯代二苯并-对-二恶英（OCS）的敏感、竞争性间接酶联免疫吸附测定法（ELISA）。为了获得最敏感的抗体，合成了几种带有不同连接子的半抗原，以模拟 OCS 的特殊结构，并将其与载体蛋白偶联。针对不同的免疫原获得了多克隆兔抗体，并针对不同的包被抗原进行了筛选。在优化条件下，这种间接 ELISA 显示出从 1.4 到 86.3ng/mL 的线性检测范围，IC50 值为 4.46ng/mL，检测限（LOD）为 0.1ng/mL。测试了 12 种化合物以计算交叉反应性，几乎所有化合物的交叉反应性都很小（<5%）。用水和血清样品中添加 OCS 进行 ELISA 分析，获得的回收率令人满意，平均回收率为 92%。这种免疫测定法可以作为一种快速、方便的工具，用于监测环境样品中的 OCS。
