State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Anal Biochem. 2014 Feb 15;447:15-22. doi: 10.1016/j.ab.2013.11.004. Epub 2013 Nov 13.
A competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for detection of tris(2,3-dibromopropyl) isocyanurate (TBC). Polyclonal antibodies against TBC were raised from synthesized haptens and then screened against various coating antigens. After optimization of the immunoassay conditions, the linear range and IC₅₀ value of the assay were 0.30-100 and 5.17 μg/L, respectively, with little cross-reactivity (≤2%). Recovery of various samples (water, serum, soil) was tested and the values ranged from 68% to 110%. This ciELISA was also applied to determine TBC in the riverside soil of the Liuyang River, and the results were compared with the data obtained by UHPLC-MS/MS. The experimental assay results confirmed that this proposed immunoassay is a specific, sensitive, and reliable method for determination and monitoring of TBC.
建立了一种竞争性间接酶联免疫吸附测定法（ciELISA）用于检测三（2,3-二溴丙基）异氰尿酸酯（TBC）。用合成的半抗原制备了针对 TBC 的多克隆抗体，然后对各种包被抗原进行筛选。在优化免疫分析条件后，该测定法的线性范围和 IC₅₀值分别为 0.30-100 和 5.17 μg/L，交叉反应性较小（≤2%）。测试了各种样品（水、血清、土壤）的回收率，回收率范围为 68%至 110%。该 ciELISA 还用于测定浏阳河河岸土壤中的 TBC，并将结果与 UHPLC-MS/MS 获得的数据进行比较。实验检测结果证实，该免疫分析方法是一种用于 TBC 检测和监测的特异性、灵敏性和可靠的方法。
