Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P.R. China.
Food Funct. 2013 Oct;4(10):1526-34. doi: 10.1039/c3fo60171d.
The purpose of this study was to analyze the influence of varieties on the constituents, antioxidant and anticancer activities of corn silk. The contents of total phenolic and flavonoids and individual flavonoids in six corn silk varieties (Denghai6702, Delinong988, Tunyu808, Zhongdan909, Liangyu208, Jingke968) were comparatively analyzed by colourimetric methods, high performance liquid chromatography (HPLC) methods and antioxidant activities were assessed using a panel of in vitro assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity assay, the inhibitory effects on lipid peroxidation (MDA) assay and ferric reducing/antioxidant power (FRAP) assay, and the cytotoxicity against human prostatic carcinoma cells PC3 and breast carcinoma cells MDA-MB-231 and MCF7 were also evaluated. Results showed that Zhongdan909 exhibited the highest total phenolic content while Tunyu808 had the highest flavonoid content among the six species. Zhongdan909 showed the highest DPPH radical scavenging activity, the highest inhibitory effect on lipid peroxidation and the strongest cytotoxicity against breast carcinoma cells MCF7, while Tunyu808 exhibited the highest reducing power. There were good relationships between the total phenolic and flavonoid contents and antioxidant activities (r > 0.78) and the cytotoxicity against breast carcinoma cells MCF7 (r > 0.79). This study suggested that corn silk could be potentially used as a readily accessible source of natural antioxidants and formononetin was one of the main antioxidant constituents in corn silk.
本研究旨在分析品种对玉米须成分、抗氧化和抗癌活性的影响。采用比色法、高效液相色谱法（HPLC）比较分析了 6 个玉米须品种（登海 6702、德林龙 988、屯玉 808、中单 909、梁玉 208、晶科 968）中总酚和类黄酮的含量以及个别类黄酮的含量，并采用一系列体外测定法评估了抗氧化活性，包括 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性测定法、抑制脂质过氧化（MDA）测定法和铁还原/抗氧化能力（FRAP）测定法，还评估了对人前列腺癌细胞 PC3 和乳腺癌细胞 MDA-MB-231 和 MCF7 的细胞毒性。结果表明，中单 909 总酚含量最高，而屯玉 808 黄酮含量最高。中单 909 对 DPPH 自由基清除活性最高，对脂质过氧化抑制作用最强，对乳腺癌细胞 MCF7 的细胞毒性最强，而屯玉 808 的还原能力最强。总酚和类黄酮含量与抗氧化活性（r > 0.78）和对乳腺癌细胞 MCF7 的细胞毒性（r > 0.79）之间存在良好的关系。本研究表明，玉米须可能是一种很容易获得的天然抗氧化剂的来源，芒柄花素是玉米须中的主要抗氧化成分之一。
