Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.
Indian J Med Res. 2013;138(1):104-10.
BACKGROUND & OBJECTIVES: BMP (bone morphogenetic protein)-4/7 and bFGF (basic fibroblast growth factor) significantly promote the osteogenic activity and the proliferation of rabbit BMSCs (bone marrow stromal cells), respectively. However, their synergistic effects on the proliferation and the differentiation of BMSCs remain unclear. In the present study, the effects of bFGF and BMP-4/7 were investigated on the proliferation and the differentiation of rat BMSCs in vitro.
BMSCs were isolated from New Zealand white rabbits and cultured to the third passage. The samples were divided into five groups according to the material implanted: (A) 80 ng/ml BMP-4/7; (B) 80 ng/ml bFGF; (C) 30 ng/ml BMP-4/7 and 30 ng/ml bFGF; (D) 50 ng/ml BMP-4/7 and 50 ng/ml bFGF; and (E) 80 ng/ml BMP-4/7 and 80 ng/ml bFGF. Cell proliferation was analyzed using methyl thiazolyl tetrazolium (MTT) assay. Alkaline phosphatase activity and osteocalcin (OC) dynamics were also measured.
BMP-4/7 alone significantly (P<0.05) promoted the proliferation of BMSCs. At the same time, it also promoted or inhibited the osteogenic differentiation of BMSCs. The synergistic effects of BMP-4/7 and bFGF significantly promoted both the proliferation and the osteogenic differentiation of BMSCs. The treatment of the synergistic effects was dose and time dependent.
INTERPRETATION & CONCLUSIONS: A rational combination of BMP-4/7 and bFGF can promote the proliferation and the osteogenic differentiation of BMSCs. In addition, the synergistic functions are effective.
BMP（骨形态发生蛋白）-4/7 和 bFGF（碱性成纤维细胞生长因子）分别显著促进兔 BMSCs（骨髓基质细胞）的成骨活性和增殖。然而，它们对 BMSCs 的增殖和分化的协同作用尚不清楚。在本研究中，研究了 bFGF 和 BMP-4/7 对体外大鼠 BMSCs 的增殖和分化的影响。
从新西兰白兔中分离 BMSCs 并培养至第三代。根据植入材料将样本分为五组：（A）80ng/ml BMP-4/7；（B）80ng/ml bFGF；（C）30ng/ml BMP-4/7 和 30ng/ml bFGF；（D）50ng/ml BMP-4/7 和 50ng/ml bFGF；和（E）80ng/ml BMP-4/7 和 80ng/ml bFGF。使用甲基噻唑基四唑（MTT）测定分析细胞增殖。还测量碱性磷酸酶活性和骨钙素（OC）动态。
BMP-4/7 单独显着（P<0.05）促进 BMSCs 的增殖。同时，它还促进或抑制 BMSCs 的成骨分化。BMP-4/7 和 bFGF 的协同作用显着促进 BMSCs 的增殖和成骨分化。协同作用的处理是剂量和时间依赖性的。
BMP-4/7 和 bFGF 的合理组合可以促进 BMSCs 的增殖和成骨分化。此外，协同功能有效。
