下一代蛋白设计。
NextGen protein design.
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06511, U.S.A.
Integrated Graduate Program in Physical and Engineering Biology, Yale University, 266 Whitney Avenue, New Haven, CT 06511, U.S.A.
出版信息
Biochem Soc Trans. 2013 Oct;41(5):1131-1136. doi: 10.1042/BST20130112.
Abstract
Protein engineering is at an exciting stage because designed protein-protein interactions are being used in many applications. For instance, three designed proteins are now in clinical trials. Although there have been many successes over the last decade, protein engineering still faces numerous challenges. Often, designs do not work as anticipated and they still require substantial redesign. The present review focuses on the successes, the challenges and the limitations of rational protein design today.
摘要
蛋白质工程正处于令人兴奋的阶段，因为设计的蛋白质-蛋白质相互作用正在许多应用中得到应用。例如，现在有三种设计的蛋白质正在临床试验中。尽管在过去的十年中有许多成功，但蛋白质工程仍然面临许多挑战。通常，设计不能按预期工作，它们仍然需要大量的重新设计。本综述重点介绍了理性蛋白质设计的今天的成功、挑战和局限性。
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