Center for Translational Musculoskeletal Research, School of Health and Rehabilitation Sciences, Indiana University , Indianapolis, IN , USA .
Connect Tissue Res. 2013;54(6):386-93. doi: 10.3109/03008207.2013.848201.
The Achilles tendon is a frequent site for degeneration, and advanced understanding of this pathology requires an animal model that replicates the human condition. The aim of this study was to explore whether intratendinous collagenase injection combined with treadmill running created a pathology in the rat Achilles tendon consistent with human Achilles tendinosis. Collagenase was injected into one Achilles tendon of 88 high-capacity running (HCR) rats, which were randomized into treadmill running and cage control groups. Running animals ran at speeds up to 30 m/min on a treadmill at a 15° incline for up to 1 h/d, 5 d/week for 4 or 10 weeks. Cage control animals maintained cage activity. Collagenase induced molecular, histopathological and mechanical changes within the Achilles tendon at 4 weeks. The mechanical changes persisted at 10 weeks; however, the histopathological and majority of the molecular changes were no longer present at 10 weeks. Treadmill running had minimal effect and did not exacerbate the collagenase-induced changes as there were no statistical interactions between the interventions. These data suggest combined intratendinous collagenase injection and treadmill running does not create pathology within the Achilles tendon of rats selectively bred for HCR that is consistent with human Achilles tendinosis.
跟腱是变性的常见部位，要深入了解这种病理学，需要建立一种能复制人类疾病的动物模型。本研究旨在探讨在大鼠跟腱内注射胶原酶并结合跑台运动是否能产生一种与人跟腱病相一致的病理变化。88 只高容量跑（HCR）大鼠的一侧跟腱内注射胶原酶，随机分为跑台运动组和笼养对照组。跑台运动组的动物在 15°倾斜的跑台上以高达 30m/min 的速度运动，每天 1 小时，每周 5 天，持续 4 或 10 周。笼养对照组的动物保持笼内活动。胶原酶在 4 周内引起跟腱内的分子、组织病理学和力学变化。在 10 周时，力学变化仍然存在；然而，组织病理学和大多数分子变化在 10 周时不再存在。跑台运动的影响很小，没有加剧胶原酶诱导的变化，因为干预之间没有统计学上的相互作用。这些数据表明，在专门用于 HCR 的大鼠的跟腱内联合注射胶原酶和跑台运动不能产生与人跟腱病一致的选择性病理学变化。
