Giwercman A, Lindenberg S, Kimber S J, Andersson T, Müller J, Skakkebaek N E
University Department of Obstetrics and Gynecology, Righospitalet, Copenhagen, Denmark.
Cancer. 1990 Mar 1;65(5):1135-42. doi: 10.1002/1097-0142(19900301)65:5<1135::aid-cncr2820650517>3.0.co;2-t.
Invasive germ cell cancer can be prevented if the neoplasia is diagnosed at the stage of carcinoma in situ (CIS). In routine histologic examination CIS may be overlooked, particularly in prepubertal individuals. The detection of this early malignancy may be greatly facilitated by use of immunohistochemical staining. The authors investigated the sensitivity of an immunohistochemical staining procedure with monoclonal antibody 43-9F in detection of CIS. Testicular specimens from 19 adult and two prepubertal individuals with CIS were tested. Positive staining reaction on the surface of malignant germ cells was encountered in all 21 specimens with CIS. The epithelial cells of the excretory ducts between testis and epididymis, including rete testis and canaliculi efferentes, reacted also positively with 43-9F. No staining was observed in nonmalignant testicular cells including Sertoli cells or Leydig cells in any of these samples or in testicular biopsy specimens from 27 adult and 11 prepubertal subjects without evidence of testicular neoplasia. Monoclonal antibody 43-9F was also found to react with cells of all four tested invasive testicular germ cell tumors. Thus, the monoclonal antibody 43-9F is a sensitive immunohistochemical marker of CIS germ cells and may also be of potential value in detection of invasive testicular cancer.
如果在原位癌（CIS）阶段诊断出肿瘤，侵袭性生殖细胞癌是可以预防的。在常规组织学检查中，CIS可能会被忽视，尤其是在青春期前个体中。使用免疫组化染色可极大地促进这种早期恶性肿瘤的检测。作者研究了用单克隆抗体43-9F进行免疫组化染色程序在检测CIS中的敏感性。对19名成年和2名患有CIS的青春期前个体的睾丸标本进行了检测。所有21份CIS标本的恶性生殖细胞表面均出现阳性染色反应。睾丸和附睾之间排泄管的上皮细胞，包括睾丸网和输出小管，对43-9F也呈阳性反应。在这些样本中的任何一个以及来自27名成年和11名青春期前无睾丸肿瘤证据的受试者的睾丸活检标本中，包括支持细胞或间质细胞在内的非恶性睾丸细胞均未观察到染色。还发现单克隆抗体43-9F与所有四种测试的侵袭性睾丸生殖细胞肿瘤的细胞发生反应。因此，单克隆抗体43-9F是CIS生殖细胞的一种敏感免疫组化标志物，在侵袭性睾丸癌的检测中也可能具有潜在价值。
