Department of Chemical and Biological Engineering, Pharmaceutical Technology, Chalmers University of Technology , SE-41296 Gothenburg, Sweden.
Langmuir. 2013 Oct 22;29(42):13058-65. doi: 10.1021/la402732a. Epub 2013 Oct 9.
The phase behavior of 1-glyceryl monoleyl ether (GME) in mixtures of water and the solvents 1,5-pentanediol (POL) or N-methyl-2-pyrrolidone (NMP) was investigated by ocular inspection, polarization microscopy, and small-angle X-ray diffraction (SAXD). Phase diagrams were constructed based on analyses of more than 200 samples prepared using the two different solvents at 20 °C. The inverse hexagonal phase formed by GME in excess of water was transformed into the cubic and sponge phase with the increasing amount of each solvent. Particularly POL allowed for the formation of an extended sponge phase area in the phase diagram, comprising up to 70% POL-water mixture. The phase behavior using NMP was found to be similar to the earlier investigated solvent propylene glycol. The extended sponge phase for the POL system was attributed to POLs strong surface/interfacial activity with the potential to stabilize the polar/apolar interface of the sponge phase. The cubic and sponge phases formed using POL were further studied by NMR in order to measure the partitioning of POL between the lipid and aqueous domains of the phases. The domain partition coefficient K (lipid domain/aqueous domain) for POL in cubic and sponge phases was found to be 0.78 ± 0.14 and constant for the two phases.
通过肉眼观察、偏光显微镜和小角 X 射线衍射（SAXD）研究了 1-甘油单油酸酯（GME）在水与溶剂 1,5-戊二醇（POL）或 N-甲基-2-吡咯烷酮（NMP）混合物中的相行为。根据在 20°C 下使用两种不同溶剂制备的超过 200 个样品的分析，构建了相图。在过量水形成的 GME 反六方相被转化为立方相和海绵相，随着每种溶剂的量的增加。特别是 POL 允许在相图中形成扩展的海绵相区，包含高达 70%的 POL-水混合物。发现使用 NMP 的相行为与早期研究的溶剂丙二醇相似。对于 POL 系统的扩展海绵相归因于 POL 具有强的表面/界面活性，有可能稳定海绵相的极性/非极性界面。通过 NMR 进一步研究了使用 POL 形成的立方相和海绵相，以测量 POL 在相的脂质和水相之间的分配。在立方相和海绵相中，POL 的域分配系数 K（脂质域/水相）为 0.78±0.14，并且对于两相是恒定的。
