College of Chemistry & Bio-engineering, Yichun University, Yichun, Jiangxi 336000, People's Republic of China.
Fitoterapia. 2013 Dec;91:298-304. doi: 10.1016/j.fitote.2013.09.009. Epub 2013 Sep 20.
The aim of the study was to investigate the anti-asthmatic effects of apigenin and the possible mechanisms. Asthma model was established by ovalbumin-induced asthma. A total of 50 mice were randomly assigned to six experimental groups: control, model, dexamethasone (2 mg/kg) and apigenin (5 mg/kg, 10 mg/kg). Airway resistance (Raw) was measured, histological studies were evaluated by hematoxylin and eosin (HE) staining, OVA-specific serum and BALF IgE levels and Th17 cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA), Th17 cells were evaluated by flow cytometry (FCM), and protein level of RORγt was measured by western blotting. Our study demonstrated that apigenin inhibited OVA-induced increases in Raw and eosinophil count; interleukin (IL)-6, TNF- and IL-17A levels were recovered. Histological studies demonstrated that apigenin substantially inhibited OVA-induced eosinophilia in lung tissue and airway tissue. Flow cytometry studies demonstrated that apigenin substantially inhibited Th17 cells. Western blotting studies demonstrated that apigenin substantially inhibited RORγt protein level. These findings suggest that apigenin may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
本研究旨在探讨芹菜素的抗哮喘作用及其可能的机制。采用卵清蛋白诱导的哮喘模型。将 50 只小鼠随机分为六组：对照组、模型组、地塞米松（2mg/kg）组和芹菜素（5mg/kg、10mg/kg）组。测定气道阻力（Raw），苏木精和伊红（HE）染色评估组织学研究，酶联免疫吸附试验（ELISA）评估 OVA 特异性血清和 BALF IgE 水平和 Th17 细胞因子，流式细胞术（FCM）评估 Th17 细胞，Western blot 法测定 RORγt 蛋白水平。我们的研究表明，芹菜素抑制 OVA 诱导的 Raw 和嗜酸性粒细胞计数增加；白细胞介素（IL）-6、TNF-α 和 IL-17A 水平恢复。组织学研究表明，芹菜素可显著抑制肺组织和气道组织中的 OVA 诱导的嗜酸性粒细胞浸润。流式细胞术研究表明，芹菜素可显著抑制 Th17 细胞。Western blot 研究表明，芹菜素可显著抑制 RORγt 蛋白水平。这些发现表明，芹菜素可能有效改善哮喘的进展，可作为治疗变应性哮喘患者的一种方法。
