West Thomas P, Peterson Jessica L
Department of Biology and Microbiology, South Dakota State University, Box 2104A, Brookings, SD, 57007, USA,
Biotechnol Lett. 2014 Jan;36(1):147-52. doi: 10.1007/s10529-013-1343-y. Epub 2013 Sep 24.
A mutant strain of Citrobacter freundii capable of elevated 3-hydroxypropionaldehyde production from glycerol was isolated using chemical mutagenesis and a screening protocol. The protocol involved screening mutagenized bacterial cells on solid minimal medium containing 5 % (v/v) glycerol. Colonies were picked onto duplicate solid minimal medium plates and one plate was stained with 1 % (w/v) phloroglucinol. Those colonies staining red were further screened and a mutant, HPAO-1, was identified. The mutant strain produced a several-fold higher 3-hydroxypropionaldehyde concentration than did the parent strain when grown on 5 % (v/v) glycerol. The ratio of culture volume to flask volume influenced 3-hydroxypropionaldehyde production by the mutant cells compared to the parent cells. Aldehyde production was highest when the mutant strain was grown on 5 % (v/v) glycerol at a ratio of culture volume to flask volume of 1:3 or 1:12.5.
利用化学诱变和筛选方案分离出了一种能够从甘油中提高3-羟基丙醛产量的弗氏柠檬酸杆菌突变株。该方案包括在含有5%（v/v）甘油的固体基本培养基上筛选诱变细菌细胞。将菌落挑到一式两份的固体基本培养基平板上，其中一个平板用1%（w/v）间苯三酚染色。将那些染成红色的菌落进一步筛选，鉴定出一个突变株HPAO-1。当在5%（v/v）甘油上生长时，该突变株产生的3-羟基丙醛浓度比亲本菌株高出几倍。与亲本细胞相比，培养体积与烧瓶体积的比例影响突变细胞产生3-羟基丙醛。当突变株在5%（v/v）甘油上以培养体积与烧瓶体积之比为1:3或1:12.5生长时，醛产量最高。
