Izon D J, Boyd R L
Department of Pathology and Immunology, Monash University Medical School, Melbourne, Australia.
Hum Immunol. 1990 Jan;27(1):16-32. doi: 10.1016/0198-8859(90)90092-4.
Seven monoclonal antibodies were produced against human thymic stromal cells. The monoclonal antibodies were put into two groups depending on whether the cells they detected were keratin-positive or -negative. Demonstrated in the keratin-negative group were a granular reticular meshwork, a stellate population predominantly in the medulla, and secretory cells associated with Hassall's corpuscles. In the keratin-positive group we showed two extensive epithelial networks, a trabecular and rare medullary epithelial population, and thymus-specific epithelium restricted to the subcapsule and the medulla. A novel finding was that some of the monoclonal antibodies and also OKM1 identified keratin-negative cells within Hassall's corpuscles, which implies that there are macrophages associated with these structures. The monoclonal antibodies should prove useful for separating and classifying subpopulations of stromal cells and also for monitoring changes in the thymic architecture in different thymic pathologies.
制备了七种针对人胸腺基质细胞的单克隆抗体。根据所检测细胞是角蛋白阳性还是阴性，将单克隆抗体分为两组。在角蛋白阴性组中可见颗粒状网状结构、主要位于髓质的星状细胞群以及与哈氏小体相关的分泌细胞。在角蛋白阳性组中，我们发现了两个广泛的上皮网络、小梁和罕见的髓质上皮细胞群，以及局限于被膜下和髓质的胸腺特异性上皮。一个新发现是，一些单克隆抗体以及OKM1在哈氏小体内鉴定出角蛋白阴性细胞，这意味着这些结构中有巨噬细胞与之相关。这些单克隆抗体应有助于分离和分类基质细胞亚群，也有助于监测不同胸腺病变中胸腺结构的变化。
