人类胸腺微环境：通过单克隆抗上皮细胞抗体检测到的异质性

de Maagd R A, MacKenzie W A, Schuurman H J, Ritter M A, Price K M, Broekhuizen R, Kater L

Immunology. 1985 Apr;54(4):745-54.

Monoclonal antibodies were raised against human thymus stromal cells and their specificity for the epithelial component of thymus stroma assessed by double immunofluorescence using anti-keratin antibodies to identify epithelium. Our monoclonal antibodies identify six distinct patterns of epithelial cell antigen expression within the thymus: pan epithelial (antibody IP1); cortex (MR3 and MR6); cortical/medullary junction (IP2); subcapsule and subpopulation of medulla (MR10/MR14); Hassall's corpuscles and adjacent subpopulation of medulla (IP3); Hassall's corpuscles only (MR13/IP4). This heterogeneity of antigen expression suggests that many different epithelial microenvironments exist within the human thymus.

制备了针对人胸腺基质细胞的单克隆抗体，并通过使用抗角蛋白抗体鉴定上皮细胞的双重免疫荧光法评估了它们对胸腺基质上皮成分的特异性。我们的单克隆抗体识别出胸腺内上皮细胞抗原表达的六种不同模式：全上皮（抗体IP1）；皮质（MR3和MR6）；皮质/髓质交界处（IP2）；被膜下和髓质亚群（MR10/MR14）；哈氏小体和相邻的髓质亚群（IP3）；仅哈氏小体（MR13/IP4）。这种抗原表达的异质性表明，人类胸腺内存在许多不同的上皮微环境。