Liu K-H, Wang C-P, Chang M-F, Chung Y-W, Lou P-J, Lin J-H
Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Department of Otolaryngology, College of Medicine, National Taiwan University Hospital and National Taiwan University, Taipei, Taiwan.
Hum Exp Toxicol. 2014 Jun;33(6):629-37. doi: 10.1177/0960327113485257. Epub 2013 Sep 24.
Photodynamic therapy (PDT) is a novel cancer treatment based on the tumor-specific accumulation of a photosensitizer followed by irradiation with visible light, which induces selective tumor cell death via production of reactive oxygen species. To elucidate the underlying mechanisms, microarray analysis was used to analyze the changes in gene expression patterns during PDT induced by various photosensitizers. Cancer cells were subjected to four different photosensitizer-mediated PDT and the resulting gene expression profiles were compared. We identified many differentially expressed genes reported previously as well as new genes for which the functionfunctions in PDT are still unclear. Our current results not only advance the general understanding of PDT but also suggest that distinct molecular mechanisms are involved in different photosensitizer-mediated PDT. Elucidating the signaling mechanisms in PDT will provide information to modulate the antitumor effectiveness of PDT using various photosensitizers.
光动力疗法（PDT）是一种新型癌症治疗方法，它基于光敏剂在肿瘤中的特异性积聚，随后用可见光照射，通过产生活性氧诱导肿瘤细胞选择性死亡。为了阐明其潜在机制，利用微阵列分析来分析各种光敏剂诱导的光动力疗法过程中基因表达模式的变化。癌细胞接受四种不同的光敏剂介导的光动力疗法，并比较所得的基因表达谱。我们鉴定出了许多先前报道的差异表达基因以及在光动力疗法中功能仍不清楚的新基因。我们目前的结果不仅推进了对光动力疗法的总体理解，还表明不同的光敏剂介导的光动力疗法涉及不同的分子机制。阐明光动力疗法中的信号传导机制将为利用各种光敏剂调节光动力疗法的抗肿瘤效果提供信息。
