基于脑脊液的 cryptococcal 抗原侧向流检测对隐球菌性脑膜炎的即时诊断和预后评估。
Point-of-care diagnosis and prognostication of cryptococcal meningitis with the cryptococcal antigen lateral flow assay on cerebrospinal fluid.
机构信息
Department of Medicine, Faculty of Medicine, Mbarara University of Science and Technology, Uganda.
出版信息
Clin Infect Dis. 2014 Jan;58(1):113-6. doi: 10.1093/cid/cit641. Epub 2013 Sep 24.
Abstract
The cryptococcal antigen (CRAG) lateral flow assay (LFA) had 100% sensitivity and specificity on cerebrospinal fluid samples. Pretreatment LFA titers correlated with quantitative cultures (R(2) = 0.7) and predicted 2- and 10-week mortality. The CRAG LFA is an accurate diagnostic assay for CSF and should be considered for point-of-care diagnosis of cryptococcal meningitis.
摘要
隐球菌抗原（CRAG）侧向流动检测（LFA）对脑脊液样本具有 100%的灵敏度和特异性。预处理 LFA 滴度与定量培养相关（R(2) = 0.7），并预测 2 周和 10 周的死亡率。CRAG LFA 是一种准确的脑脊液诊断检测方法，应考虑用于隐球菌性脑膜炎的即时诊断。
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2
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3
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Clin Vaccine Immunol. 2013 Jan;20(1):52-5. doi: 10.1128/CVI.00536-12. Epub 2012 Oct 31.
4
Short course amphotericin B with high dose fluconazole for HIV-associated cryptococcal meningitis.短程两性霉素 B 联合高剂量氟康唑治疗 HIV 相关隐球菌性脑膜炎。
J Infect. 2012 Jan;64(1):76-81. doi: 10.1016/j.jinf.2011.10.014. Epub 2011 Nov 4.
5
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PLoS Med. 2010 Dec 21;7(12):e1000384. doi: 10.1371/journal.pmed.1000384.
6
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