Zhang W Q, Zhang M H
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang Province, China.
Genet Mol Res. 2013 Sep 3;12(3):3256-62. doi: 10.4238/2013.September.3.1.
Many mitochondrial DNA sequences are used to estimate phylogenetic relationships among animal taxa and perform molecular phylogenetic evolution analysis. With the continuous development of sequencing technology, numerous mitochondrial sequences have been released in public databases, especially complete mitochondrial DNA sequences. Using multiple sequences is better than using single sequences for phylogenetic analysis of animals because multiple sequences have sufficient information for evolutionary process reconstruction. Therefore, we performed phylogenetic analyses of 14 species of Felidae based on complete mitochondrial genome sequences, with Canis familiaris as an outgroup, using neighbor joining, maximum likelihood, maximum parsimony, and Bayesian inference methods. The consensus phylogenetic trees supported the monophyly of Felidae, and the family could be divided into 2 subfamilies, Felinae and Pantherinae. The genus Panthera and species tigris were also studied in detail. Meanwhile, the divergence of this family was estimated by phylogenetic analysis using the Bayesian method with a relaxed molecular clock, and the results shown were consistent with previous studies. In summary, the evolution of Felidae was reconstructed by phylogenetic analysis based on mitochondrial genome sequences. The described method may be broadly applicable for phylogenetic analyses of anima taxa.
许多线粒体DNA序列被用于估计动物类群之间的系统发育关系，并进行分子系统发育进化分析。随着测序技术的不断发展，公共数据库中已发布了大量线粒体序列，尤其是完整的线粒体DNA序列。在动物的系统发育分析中，使用多条序列比使用单条序列更好，因为多条序列具有足够的信息用于重建进化过程。因此，我们以家犬为外类群，基于完整的线粒体基因组序列，使用邻接法、最大似然法、最大简约法和贝叶斯推断法，对14种猫科动物进行了系统发育分析。一致的系统发育树支持猫科的单系性，该科可分为两个亚科，即猫亚科和豹亚科。同时，对豹属和虎种也进行了详细研究。此外，使用具有宽松分子钟的贝叶斯方法通过系统发育分析估计了该科的分歧时间，结果与先前的研究一致。总之，基于线粒体基因组序列的系统发育分析重建了猫科的进化历程。所描述的方法可能广泛适用于动物类群的系统发育分析。
