Novello M, Rodrigues J F, Pinheiro F, Oliveira G C X, Veasey E A, Koehler S
Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
Genet Mol Res. 2013 Sep 3;12(3):3274-8. doi: 10.4238/2013.September.3.3.
Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petrópolis population. The mean number of alleles per locus was 8.8 between populations. The mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species.
为濒危的巴西兰花物种科氏卡特兰开发了微卫星标记，以描述其遗传多样性和结构，并支持保护研究。使用富集基因组文库分离并鉴定了9个微卫星位点。除了彼得罗波利斯种群的Cac05和Cac09位点外，所有位点在至少两个采样种群中都是多态的。种群间每个位点的平均等位基因数为8.8。观察到的杂合度和预期杂合度的平均值分别为0.541（范围从0到1）和0.639（范围从0到0.9）。在另外7种附生兰科植物中进行了交叉扩增，在另外3种卡特兰属植物、二列树兰和细叶巴西兰中至少有2个位点成功扩增。本文所述的所有标记将有助于进一步评估科氏卡特兰及相关物种的遗传多样性、种群动态和保护遗传学。
