Hermann Katia L, Costa Alessandra, Helm Cristiane V, De Lima Edson A, Tavares Lorena B B
Universidade Regional de Blumenau, 89030-000 Blumenau SC, Brasil.
An Acad Bras Cienc. 2013 Sep;85(3):965-73. doi: 10.1590/S0001-37652013000300009.
The production of ethanol from lignocellulosic biomass is referred as a second generation biofuel, whose processing is one of the most promising technologies under development. There are few available studies on the use of enzymes produced by fungi as active for the biodegradation of lignocellulosic biomass. However, the manganese peroxidase (MnP) enzyme presents high potential to degrade lignin and the basidiomycetes are the major producers of this oxidase. Thus, this study aimed at evaluating the ability of fungi Lentinula edodes and Lentinula boryana to produce this enzyme when cultivated in submerged fermentation system (SS) and also in solid-state fermentation system (SSF) containing Eucalyptus benthamii sawdust with or without corn cob meal. In the SS the greatest MnP expression occurred on the 25th day, being of 70 UI.L-1 for L. boryana and of 20 UI.L-1 for L. edodes. In the SSF, the best results were obtained on the 10th day for L. edodes, while for L. boryana it happened between the 20th and the 25th days, despite both species presented values close to 110 UI.L-1. Therefore, the results indicated that the studied fungi express the enzyme of interest and that its production is enhanced when cultivated in solid system.
从木质纤维素生物质生产乙醇被称为第二代生物燃料，其加工是目前正在开发的最具前景的技术之一。关于真菌产生的酶对木质纤维素生物质生物降解具有活性的研究很少。然而，锰过氧化物酶（MnP）具有降解木质素的高潜力，担子菌是这种氧化酶的主要生产者。因此，本研究旨在评估香菇和鲍氏香菇在含有或不含有玉米芯粉的本氏桉木屑的深层发酵系统（SS）和固态发酵系统（SSF）中培养时产生这种酶的能力。在深层发酵系统中，最大的MnP表达出现在第25天，鲍氏香菇为70 UI.L-1，香菇为20 UI.L-1。在固态发酵系统中，香菇在第10天获得了最佳结果，而鲍氏香菇在第20天至第25天之间获得了最佳结果，尽管两个物种的值都接近110 UI.L-1。因此，结果表明所研究的真菌表达了目标酶，并且在固体系统中培养时其产量会提高。
