Lu Xiaohong, Zhao Yuanyuan, Li Fei, Liu Ping
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Int J Biol Macromol. 2023 Feb 15;228:647-658. doi: 10.1016/j.ijbiomac.2022.12.264. Epub 2022 Dec 28.
In this study, Lentinula edodes and Pleurotus ostreatus were selected as representatives to explore the effects of corn straw and xylosma sawdust on the production and activity of polysaccharides by edible fungi during solid-state fermentation. The results clearly indicated that the addition of lignocellulose could promote the polysaccharide content compared to the control group. Meanwhile, the hydroxyl radical scavenging activity of polysaccharides reached the maximum when the glucose concentration was 1.5 %, and among them, the xylosma sawdust groups for two fungi (L. edodes-32.37 %, P. ostreatus-25.86 %) both performed better than corn straw groups (L. edodes-24.96 %, P. ostreatus-20.80 %). In addition, structural characterization and degradation mode analysis were carried out. The results showed that the structure of the xylosma had a stronger destruction than corn straw. The activities of lignocellulolytic enzymes such as carboxymethyl cellulase, filter paper enzyme, β-glucosidase and xylanase increased rapidly in the early stage of fermentation, which could degrade the cellulose and hemicellulose as raw materials for the synthesis of active polysaccharides.
在本研究中，选择香菇和平菇作为代表，以探究玉米秸秆和柞木屑对食用菌在固态发酵过程中多糖产量和活性的影响。结果清楚地表明，与对照组相比，添加木质纤维素可提高多糖含量。同时，当葡萄糖浓度为1.5%时，多糖的羟基自由基清除活性达到最大值，其中，两种真菌（香菇-32.37%，平菇-25.86%）的柞木屑组均比玉米秸秆组（香菇-24.96%，平菇-20.80%）表现更好。此外，还进行了结构表征和降解模式分析。结果表明，柞木屑的结构比玉米秸秆受到的破坏更强。羧甲基纤维素酶、滤纸酶、β-葡萄糖苷酶和木聚糖酶等木质纤维素分解酶的活性在发酵初期迅速增加，这些酶可将纤维素和半纤维素降解，作为合成活性多糖的原料。
