Department of Pediatrics, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Arch Virol. 2014 Mar;159(3):499-507. doi: 10.1007/s00705-013-1773-9. Epub 2013 Sep 26.
This study reports the molecular epidemiology and genetic characterization of human respiratory syncytial virus (RSV) samples collected in Thailand from January 2010 to December 2011. In total, 1,315 clinical samples were collected from Bangkok and Khon Kaen provinces and were screened by semi-nested PCR for RSV infection. We found 74 samples (27.7 %) and 71 samples (6.8 %) to be RSV positive for Bangkok and Khon Kaen, respectively, and we sequenced 122 of these samples. Phylogenetic analysis revealed that 100 of the RSV-A-positive samples clustered into either genotype NA1 or the recently discovered genotype ON1 strain, which has a 72-nucleotide duplication in the second variable region of its G protein. Moreover, 22 of the RSV-B-positive samples clustered into four genotypes; BA4, BA9, BA10 and genotype THB, first described here. The NA1 genotype was found to be the predominant strain in 2010 and 2011. The ON1 strain detected in this study first emerged in 2011 and is genetically similar to ON1 strains characterized in other counties. We also describe the THB genotype, which was first identified in 2005 and is genetically similar to the GB2 genotype. In conclusion, this study indicates the importance of molecular epidemiology and characterization of RSV in Thailand in order to better understand this virus. Further studies should be conducted to bolster the development of antiviral agents and a vaccine.
本研究报告了 2010 年 1 月至 2011 年 12 月期间在泰国收集的人类呼吸道合胞病毒（RSV）样本的分子流行病学和遗传特征。总共从曼谷和孔敬省收集了 1315 份临床样本，并通过半巢式 PCR 对 RSV 感染进行了筛查。我们发现曼谷和孔敬的 RSV 阳性样本分别为 74 份（27.7%）和 71 份（6.8%），并对其中的 122 份进行了测序。系统进化分析显示，100 份 RSV-A 阳性样本聚类为 NA1 基因型或最近发现的 ON1 株，后者在其 G 蛋白的第二可变区有 72 个核苷酸的重复。此外，22 份 RSV-B 阳性样本聚类为 4 种基因型；BA4、BA9、BA10 和这里首次描述的基因型 THB。NA1 基因型是 2010 年和 2011 年的主要流行株。本研究中检测到的 ON1 株于 2011 年首次出现，与其他国家鉴定的 ON1 株在遗传上相似。我们还描述了 THB 基因型，该基因型于 2005 年首次鉴定，与 GB2 基因型在遗传上相似。总之，本研究表明在泰国进行 RSV 的分子流行病学和特征分析的重要性，以便更好地了解这种病毒。应进一步开展研究，以支持抗病毒药物和疫苗的开发。
