Department of Gastrointestinal Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Med Oncol. 2013 Dec;30(4):714. doi: 10.1007/s12032-013-0714-5. Epub 2013 Sep 26.
The aim of this study was to evaluate the expression and role of Grhl2 in gastric cancer. Immunohistochemistry was performed to explore the expression of Grhl2 in gastric cancer and surrounding non-tumor tissues. Moreover, the mRNA and protein expression level of Grhl2 in human immortalized gastric epithelial cell line GES-1 and four gastric cancer cell lines (MGC803, SGC7901, MKN45, HGC27) were detected by qRT-PCR and Western blotting, respectively. To further investigate the role of Grhl2 in gastric cancer as well as the potential mechanisms, SGC7901 cells were transfected with lentiviral constructs expressing Grhl2 or empty vector, and then proliferation and apoptosis of SGC7901 cells were evaluated by MTT assay and flow cytometry, respectively. Finally, the protein expression level of c-Myc and Bcl-2 was detected by Western blotting. Both mRNA and protein expression level of Grhl2 were significantly downregulated in gastric cancer. Exogenous Grhl2 transduced into SGC7901 cells significantly inhibited the proliferation and promoted apoptosis. Meanwhile, over-expression of Grhl2 decreased c-Myc and Bcl-2 protein expression level. Taken together, our results demonstrated that Grhl2 downregulated in gastric cancer and may function as a tumor suppressor and play an important role in the development and progression of gastric cancer. These results may provide a new clue for treatment for gastric cancer.
本研究旨在评估 Grhl2 在胃癌中的表达和作用。通过免疫组织化学方法探讨 Grhl2 在胃癌及周围非肿瘤组织中的表达。此外，通过 qRT-PCR 和 Western blot 分别检测人永生化胃上皮细胞系 GES-1 和四种胃癌细胞系（MGC803、SGC7901、MKN45、HGC27）中 Grhl2 的 mRNA 和蛋白表达水平。为了进一步研究 Grhl2 在胃癌中的作用及其潜在机制，将慢病毒构建体表达 Grhl2 或空载转染 SGC7901 细胞，然后通过 MTT assay 和流式细胞术分别评估 SGC7901 细胞的增殖和凋亡。最后，通过 Western blot 检测 c-Myc 和 Bcl-2 的蛋白表达水平。Grhl2 的 mRNA 和蛋白表达水平在胃癌中均显著下调。外源性 Grhl2 转染 SGC7901 细胞后显著抑制增殖并促进凋亡。同时，Grhl2 的过表达降低了 c-Myc 和 Bcl-2 蛋白的表达水平。综上所述，我们的研究结果表明，Grhl2 在胃癌中下调，可能作为一种肿瘤抑制因子，在胃癌的发生和发展中发挥重要作用。这些结果可能为胃癌的治疗提供新的线索。
