Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven , Kasteelpark Arenberg 20 Box 2463, B-3001 Leuven, Belgium.
J Agric Food Chem. 2013 Oct 23;61(42):10173-82. doi: 10.1021/jf403160x. Epub 2013 Oct 15.
To investigate the antioxidant capacity of ferulic acid (FA) in conjunction with prebiotic arabinoxylanoligosaccharides (AXOS), procedures for the production of FA-enriched, -depleted and cross-linked AXOS were developed, and samples were analyzed using the Trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. Results showed that not only the level of FA but also the condition under which it appears (free, bound, or dimerized) impacts the antioxidant capacity of FA-containing AXOS samples. Although esterification of FA on AXOS and cross-linking of AXOS through dehydrodiferulic acid formation lowered the antioxidant capacity of FA by 30 and 55%, respectively, as determined with the TEAC test, the antioxidant capacity of these components still remained high compared to Trolox, a water-soluble vitamin E analog. Total antioxidant capacity of the AXOS samples determined by the ORAC assay resulted in less prominent differences between the different forms of FA than those seen with the TEAC test. Feruloylated AXOS can hence function as strong, water-soluble antioxidants.
为了研究阿魏酸（FA）与益生元阿拉伯木聚糖低聚糖（AXOS）的抗氧化能力，我们开发了生产富 FA、缺 FA 和交联 AXOS 的方法，并使用 Trolox 等效抗氧化能力（TEAC）和氧自由基吸收能力（ORAC）测定法对样品进行了分析。结果表明，不仅 FA 的水平，而且 FA 出现的情况（游离、结合或二聚化）都会影响含 FA 的 AXOS 样品的抗氧化能力。尽管通过 AXOS 上的 FA 酯化和通过脱氢二阿魏酸形成交联使 FA 的抗氧化能力分别降低了 30%和 55%，但与水溶性维生素 E 类似物 Trolox 相比，这些成分的抗氧化能力仍然很高。ORAC 测定法测定的 AXOS 样品的总抗氧化能力在不同形式的 FA 之间产生的差异不如 TEAC 测试明显。因此，阿魏酰化 AXOS 可以作为强水溶性抗氧化剂。
