Chen Hong, Chen Zhuoyun, Fu Yuanfang, Liu Jiao, Lin Siying, Zhang Qing, Liu Yuntao, Wu Dingtao, Lin Derong, Han Guoquan, Wang Lina, Qin Wen
Department of Food Quality and Safety, College of Food Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China.
Department of Food Quality and Safety, Institute of Food and Drug Inspection, Chengdu 610000, Sichuan, China.
Antioxidants (Basel). 2019 Nov 25;8(12):584. doi: 10.3390/antiox8120584.
Different methods of isolating arabinoxylans (AXs) from triticale were performed to investigate the extraction methods' effects on the physiological functions of the AXs. Structural, antioxidant, and hypoglycemic activities were determined. The molecular weights (MWs) of enzyme- or water-extracted AXs were lower than those of alkali-extracted AXs. Opposite trends were shown by the arabinose-xylose ratio. Enzyme-extracted AXs exhibited higher glucose adsorption capacity and hydroxyl radical-scavenging efficiency than alkali-extracted AXs. The α-amylase inhibition ability, DPPH radical-scavenging capacity, and metal-chelating activity of alkali-extracted AXs were higher than those of enzyme-extracted AXs. Water-extracted AXs had the highest glucose dialysis retardation index. In conclusion, extraction methods can influence the physiological function of AXs through their structural features. AXs with higher MWs and esterified ferulic acid (FA) levels had higher antioxidant ability, whereas AXs with higher solubility and free FA level exhibited higher hypoglycemic activity.
采用不同方法从小黑麦中分离阿拉伯木聚糖（AXs），以研究提取方法对AXs生理功能的影响。测定了其结构、抗氧化和降血糖活性。酶法或水法提取的AXs的分子量低于碱法提取的AXs。阿拉伯糖与木糖的比例呈现相反趋势。酶法提取的AXs比碱法提取的AXs表现出更高的葡萄糖吸附能力和羟自由基清除效率。碱法提取的AXs的α-淀粉酶抑制能力、DPPH自由基清除能力和金属螯合活性高于酶法提取的AXs。水法提取的AXs具有最高的葡萄糖透析延迟指数。总之，提取方法可通过其结构特征影响AXs的生理功能。分子量较高且阿魏酸（FA）酯化水平较高的AXs具有较高的抗氧化能力，而溶解度较高且游离FA水平较高的AXs表现出较高的降血糖活性。
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