1 Department of Medical Microbiology, Abant Izzet Baysal University School of Medicine , Bolu, Turkey .
J Ocul Pharmacol Ther. 2013 Dec;29(10):893-9. doi: 10.1089/jop.2013.0120. Epub 2013 Sep 26.
We aimed to compare the efficacy of topical daptomycin (DAP) with that of vancomycin (VA) in the treatment of keratitis caused by methicillin-resistant Staphylococcus aureus (MRSA).
One hundred colony-forming unit MRSA bacteria were injected intrastromally into both corneas of 28 rabbits. Sixteen hours after injection, the rabbits' eyes were treated with 1 drop of topical DAP (10 or 50 mg/mL), VA (50 mg/mL), or isotonic saline for 19 doses. Their eyes were examined for clinical severity before and after treatment.
The minimum inhibitory concentration values of VA and DAP against the bacterial strain were found to be 2 and 0.5 μg/mL, respectively. The mean pre- and post-treatment clinical scores of the eyes did not differ significantly among the groups. However, the mean difference between the post- and pretreatment clinical scores was significantly lower in the 50 mg/mL DAP group than in the other groups (P=0.042). A marked decrease in bacterial load was detected in all treatment groups compared to the control group (P=0.002). Although there were no significant differences in bacterial load among the treatment groups, the 50 mg/mL DAP group showed the greatest decrease. The mean % epithelial erosion rate tended to be higher in the 50 mg/mL VA group than in the other groups (P=0.31).
Topical DAP significantly reduced the bacterial load and showed activity against MRSA comparable to that of fortified VA in this experimental model.
我们旨在比较局部应用达托霉素（DAP）与万古霉素（VA）治疗耐甲氧西林金黄色葡萄球菌（MRSA）性角膜炎的疗效。
将 100 个菌落形成单位的 MRSA 细菌注入 28 只兔子的双眼基质内。注射后 16 小时，用 1 滴局部 DAP（10 或 50mg/mL）、VA（50mg/mL）或等渗盐水对兔子的眼睛进行 19 次治疗。在治疗前后对其眼睛的临床严重程度进行检查。
VA 和 DAP 对该细菌株的最低抑菌浓度值分别为 2 和 0.5μg/mL。各组治疗前后的平均临床评分无显著差异。然而，与其他组相比，50mg/mL DAP 组的治疗后与治疗前临床评分的平均差异显著降低（P=0.042）。与对照组相比，所有治疗组的细菌负荷均显著降低（P=0.002）。尽管治疗组之间的细菌负荷无显著差异，但 50mg/mL DAP 组的细菌负荷降低最大。50mg/mL VA 组的平均%上皮侵蚀率趋于高于其他组（P=0.31）。
在该实验模型中，局部应用 DAP 可显著降低细菌负荷，并且对 MRSA 的活性与强化 VA 相当。
