Department of Chemical Engineering, Aristotle University of Thessaloniki, PO Box 1520, 54006 Thessaloniki, Greece.
Ecotoxicol Environ Saf. 2013 Dec;98:210-8. doi: 10.1016/j.ecoenv.2013.08.018. Epub 2013 Sep 23.
The objective of this study was the investigation of the potential use of protistan species as quality indicators of the activated sludge performance in sequential batch processes receiving toxic compounds. Two laboratory scale sequential batch reactors (SBR) were used, a conventional one and a system with plastic biofilm carriers (SBBR), treating wastewater containing phenol at concentrations ranging from 1 up to 40 mg/L. Physicochemical analyses of the samples included the determination of MLSS, effluent suspended solids, BOD5, nitrogen-ammonia, nitrogen-nitrate and phenol. The activated sludge protistan community was identified and enumerated in each reactor. Statistical analyses included Canonical Correspondence Analysis and Indicator Species Analysis of the collected experimental data. Canonical Correspondence Analysis showed inversely proportional relationships between the protozoa and the physicochemical parameters of the effluent as well as protozoan species competition. Indicator species analysis revealed the presence and the prevalence of different species under various phenol influent concentrations. No indicator species were observed for the period of operation under 5 mg/L influent phenol in both reactors, while no indicator species were observed for 20 mg/L influent phenol in the SBR reactor. Carchesium and Epistylis sp. showed the higher values for 1 mg/L phenol in the SBR, while Holophrya sp. showed lower indicator values for the same period in the SBBR. Although several species showed a good correlation to the treatment efficiency of the reactors, Blepharisma sp., could be used as the primary indicator species in both reactors for the operation period under 40 mg/L phenol, as deduced by statistical analysis.
本研究的目的是调查原生动物物种作为在连续批次处理过程中活性污泥性能质量指标的潜力，该过程中接受有毒化合物。使用了两个实验室规模的序批式反应器（SBR），一个是常规的，另一个是带有塑料生物膜载体的系统（SBBR），处理含有酚的废水，浓度范围为 1 至 40mg/L。样品的理化分析包括测定 MLSS、出水悬浮固体、BOD5、氨氮、硝酸盐氮和酚。在每个反应器中确定和计数活性污泥原生动物群落。统计分析包括对收集的实验数据进行典范对应分析和指示物种分析。典范对应分析表明，原生动物与出水的理化参数之间呈反比关系，以及原生动物物种之间的竞争。指示物种分析表明，在不同的酚类进水浓度下，不同物种的存在和流行。在两个反应器中，进水酚浓度为 5mg/L 时，没有观察到指示物种，而在 SBR 反应器中，进水酚浓度为 20mg/L 时，也没有观察到指示物种。在 SBR 中，Carchesium 和 Epistylis sp. 在 1mg/L 酚时表现出较高的值，而 Holophrya sp. 在 SBBR 中同一时期表现出较低的指示值。尽管有几个物种与反应器的处理效率有很好的相关性，但通过统计分析，Blepharisma sp.可以作为两个反应器在 40mg/L 酚运行期间的主要指示物种。
