Gu Yun, Xue Chenbin, Zhu Jianbin, Sun Hualin, Ding Fei, Cao Zheng, Gu Xiaosong
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS, 226001, People's Republic of China.
J Mol Neurosci. 2014 Apr;52(4):538-51. doi: 10.1007/s12031-013-0109-2. Epub 2013 Sep 27.
Considerable research has been devoted to unraveling the regulation of neural stem cell (NSC) differentiation. The responses of NSCs to various differentiation-inducing stimuli, however, are still difficult to estimate. In this study, we aimed to search for a potent growth factor that was able to effectively induce differentiation of NSCs toward Schwann cells. NSCs were isolated from dorsal root ganglia (DRGs) of adult rats and identified by immunostaining. Three different growth factors were used to stimulate the differentiation of DRG-derived NSCs (DRG-NSCs). We found that among these three growth factors, bFGF was the strongest inducer for the glial differentiation of DRG-NSCs, and bFGF induced the generation of an increased number of Schwann cell-like cells as compared to nerve growth factor (NGF) and neuregulin1-β (NRG). These Schwann cell-like cells demonstrated the same characteristics as those of primary Schwann cells. Furthermore, we noted that bFGF-induced differentiation of DRG-NSCs toward Schwann cells might be mediated by binding to fibroblast growth factor receptor-1 (FGFR-1) through activation of MAPK/ERK signal pathway.
大量研究致力于揭示神经干细胞（NSC）分化的调控机制。然而，NSC对各种分化诱导刺激的反应仍难以评估。在本研究中，我们旨在寻找一种能够有效诱导NSC向施万细胞分化的强效生长因子。从成年大鼠的背根神经节（DRG）中分离出NSC，并通过免疫染色进行鉴定。使用三种不同的生长因子来刺激DRG来源的NSC（DRG-NSC）的分化。我们发现，在这三种生长因子中，bFGF是DRG-NSC胶质分化的最强诱导剂，与神经生长因子（NGF）和神经调节蛋白1-β（NRG）相比，bFGF诱导产生了更多数量的施万细胞样细胞。这些施万细胞样细胞表现出与原代施万细胞相同的特征。此外，我们注意到bFGF诱导DRG-NSC向施万细胞的分化可能是通过激活MAPK/ERK信号通路与成纤维细胞生长因子受体-1（FGFR-1）结合来介导的。
