Department of Anesthesia, Children's Hospital, Fudan University, Shanghai, 201102, People's Republic of China.
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai, 200040, People's Republic of China.
Neurochem Res. 2018 Feb;43(2):375-386. doi: 10.1007/s11064-017-2432-4. Epub 2017 Nov 8.
Vinorine is a monoterpenoid indole alkaloid, a type of natural alkaloids. Growing reports exhibited the numerous pharmacology activities of vinorine such as anti-inflammation, anti-bacterial and anti-tumor. In this study, the effect of vinorine injection (7.5, 15 and 30 mg/kg) on motor function, sensation and nerve regeneration in sciatic nerve crush injury rat was investigated. The results of behavioral analysis, electrophysiological analysis and muscle histological analysis suggested that vinorine promoted the motor function recovery after sciatic nerve injury. The results of mechanical withdrawal thresholds assay and hot plate test demonstrated that vinorine improved the sensation recovery after sciatic nerve injury. The results of Fluoro-gold retrograde labeling, transmission electron microscope assay, toluidine blue and HE staining showed that vinorine attenuated the nerve damage caused by sciatic nerve injury and promoted the nerve regeneration. Furthermore, nerve growth factor (NGF) and its downstream extracellular signal-regulated kinase (ERK) signaling pathway participated in the neuro-recovery effect of vinorine after crush. In conclusion, vinorine treatment accelerated the sciatic nerve regeneration, motor function recovery and sensation recovery after crush injury via regulation of NGF and ERK activity. These results suggested that vinorine is a promising agent for never injury therapy.
文多灵是一种单萜吲哚生物碱，是天然生物碱的一种。越来越多的报道表明文多灵具有多种药理学活性，如抗炎、抗菌和抗肿瘤。在这项研究中，研究了文多灵注射液（7.5、15 和 30mg/kg）对坐骨神经挤压伤大鼠运动功能、感觉和神经再生的影响。行为分析、电生理分析和肌肉组织学分析的结果表明，文多灵促进了坐骨神经损伤后的运动功能恢复。机械撤回阈值测定和热板试验的结果表明，文多灵改善了坐骨神经损伤后的感觉恢复。荧光金逆行标记、透射电镜分析、甲苯胺蓝和 HE 染色的结果表明，文多灵减轻了坐骨神经损伤引起的神经损伤，促进了神经再生。此外，神经生长因子（NGF）及其下游细胞外信号调节激酶（ERK）信号通路参与了文多灵挤压后神经恢复的作用。总之，文多灵通过调节 NGF 和 ERK 活性，加速了挤压伤后坐骨神经的再生、运动功能的恢复和感觉的恢复。这些结果表明，文多灵是一种有前途的神经损伤治疗药物。
