Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China.
BMC Genomics. 2013 Sep 28;14:662. doi: 10.1186/1471-2164-14-662.
Chrysanthemum is one of the most important ornamental crops in the world and drought stress seriously limits its production and distribution. In order to generate a functional genomics resource and obtain a deeper understanding of the molecular mechanisms regarding chrysanthemum responses to dehydration stress, we performed large-scale transcriptome sequencing of chrysanthemum plants under dehydration stress using the Illumina sequencing technology.
Two cDNA libraries constructed from mRNAs of control and dehydration-treated seedlings were sequenced by Illumina technology. A total of more than 100 million reads were generated and de novo assembled into 98,180 unique transcripts which were further extensively annotated by comparing their sequencing to different protein databases. Biochemical pathways were predicted from these transcript sequences. Furthermore, we performed gene expression profiling analysis upon dehydration treatment in chrysanthemum and identified 8,558 dehydration-responsive unique transcripts, including 307 transcription factors and 229 protein kinases and many well-known stress responsive genes. Gene ontology (GO) term enrichment and biochemical pathway analyses showed that dehydration stress caused changes in hormone response, secondary and amino acid metabolism, and light and photoperiod response. These findings suggest that drought tolerance of chrysanthemum plants may be related to the regulation of hormone biosynthesis and signaling, reduction of oxidative damage, stabilization of cell proteins and structures, and maintenance of energy and carbon supply.
Our transcriptome sequences can provide a valuable resource for chrysanthemum breeding and research and novel insights into chrysanthemum responses to dehydration stress and offer candidate genes or markers that can be used to guide future studies attempting to breed drought tolerant chrysanthemum cultivars.
菊花是世界上最重要的观赏作物之一，干旱胁迫严重限制了其生产和分布。为了生成功能基因组学资源并深入了解菊花对脱水胁迫的分子机制，我们使用 Illumina 测序技术对菊花植物在脱水胁迫下的大规模转录组进行了测序。
我们使用 Illumina 技术对对照和脱水处理的幼苗的 mRNA 构建了两个 cDNA 文库。共生成了超过 1 亿个读数，并通过从头组装成 98180 个独特的转录本，通过将其测序与不同的蛋白质数据库进行比较，进一步广泛注释。从这些转录序列预测了生化途径。此外，我们对菊花的脱水处理进行了基因表达谱分析，鉴定了 8558 个脱水响应的独特转录本，包括 307 个转录因子和 229 个蛋白激酶以及许多众所周知的应激响应基因。GO 术语富集和生化途径分析表明，脱水胁迫导致激素反应、次生和氨基酸代谢以及光和光周期反应的变化。这些发现表明，菊花植物的耐旱性可能与激素生物合成和信号转导的调节、氧化损伤的减少、细胞蛋白和结构的稳定以及能量和碳供应的维持有关。
我们的转录组序列可以为菊花的培育和研究提供宝贵的资源，并深入了解菊花对脱水胁迫的反应，提供可用于指导未来培育耐旱菊花品种的候选基因或标记。
