Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China.
Nanoscale Res Lett. 2013 Sep 27;8(1):401. doi: 10.1186/1556-276X-8-401.
We used a baculovirus expression system to express fusion proteins of HCV core, RGD (Arg-Gly-Asp) peptide, and IFN-α2a fragments in Sf9 cells. Western blotting and electron microscopy demonstrate that HCV core, peptides RGD, and IFN-α2a fusion proteins assemble into 30 to 40 nm nano-particles (virus-like particles, VLPs). Xenograft assays show that VLPs greatly reduced tumor volume and weight with regard to a nontreated xenograft. Migration and invasion results show that VLPs can inhibit the migration and invasion of the breast cancer cells MDA-MB231. This study will provide theoretical and experimental basis for the establishment of safe and effective tumor-targeted drug delivery systems and clinical application of VLPs carrying cell interacting cargo.
我们使用杆状病毒表达系统在 Sf9 细胞中表达 HCV 核心、RGD（精氨酸-甘氨酸-天冬氨酸）肽和 IFN-α2a 片段的融合蛋白。Western blot 和电子显微镜显示，HCV 核心、肽 RGD 和 IFN-α2a 融合蛋白组装成 30 到 40nm 的纳米颗粒（病毒样颗粒，VLPs）。移植瘤实验表明，VLPs 可显著降低未经处理的移植瘤的肿瘤体积和重量。迁移和侵袭结果表明，VLPs 可以抑制乳腺癌细胞 MDA-MB231 的迁移和侵袭。本研究将为建立安全有效的肿瘤靶向药物传递系统和携带细胞相互作用货物的 VLPs 的临床应用提供理论和实验基础。
