Department of Human Movement Studies, Xi'an Physical Education University, Xi'an City, Shaanxi Province 710068, People's Republic of China.
Biomaterials. 2013 Dec;34(38):9926-36. doi: 10.1016/j.biomaterials.2013.09.018. Epub 2013 Sep 24.
Injectable calcium phosphate cement (ICPC) has been applied to enhance the tendon-to-bone healing. However, its slow degradation delays the osteointegration of grafted tendon in bone tunnels. We therefore constructed a synthetic biomaterial of ICPC combined with recombined bone xenograft granules (RBX). In this study, the first stage study demonstrated that the ICPCB contained 3 mg BMPs (ICPCB-3) obtained a porous structure. More importantly, the values of ICPCB-3 were highest in cell proliferation, alkaline phosphatase (ALP) activity, expression of osteogenic genes, and newly ectopic bone-forming area (P < 0.05). Then, ICPCB-3 was used in an anterior cruciate ligament (ACL) reconstruction model. Ninety skeletal mature rabbits underwent bilateral ACL reconstructions and were assigned to 3 groups: control group, ICPC alone group, and ICPCB-3 group. Animals were sacrificed at 6, 12 and 24 weeks. The results showed compared with ICPC, ICPCB-3 composite markedly accelerated tendon-to-bone healing. In addition, little remnants were observed in ICPCB-3 group. Moreover, the maximum loads to failure of ICPCB-3 group was significantly higher than ICPC group at 24 weeks (P < 0.01). We conclude that the ICPCB composite, with a porous structure and better osteointegration effect, has direct clinical instruction to arthroscopic techniques of the ACL reconstruction.
注射型磷酸钙骨水泥（ICPC）已被应用于增强肌腱-骨愈合。然而，其缓慢的降解延迟了移植物肌腱在骨隧道中的骨整合。因此，我们构建了一种由 ICPC 与重组异种骨移植物颗粒（RBX）复合而成的合成生物材料。在本研究中，第一阶段研究表明，含有 3mgBMP 的 ICPCB（ICPCB-3）获得了多孔结构。更重要的是，ICPCB-3 在细胞增殖、碱性磷酸酶（ALP）活性、成骨基因表达和新异位骨形成面积方面的值最高（P<0.05）。然后，将 ICPCB-3 用于前交叉韧带（ACL）重建模型。90 只骨骼成熟的兔子接受双侧 ACL 重建，并分为 3 组：对照组、ICPC 组和 ICPCB-3 组。动物在 6、12 和 24 周时处死。结果表明，与 ICPC 相比，ICPCB-3 复合材料明显加速了肌腱-骨愈合。此外，在 ICPCB-3 组中观察到的残余物较少。此外，在 24 周时，ICPCB-3 组的失效最大负载明显高于 ICPC 组（P<0.01）。我们得出结论，具有多孔结构和更好的骨整合效果的 ICPCB 复合材料对 ACL 重建的关节镜技术具有直接的临床指导意义。
