Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, BP 1173-3038, Tunisia; Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, BP 1173-3038, Tunisia.
Int J Biol Macromol. 2013 Nov;62:433-8. doi: 10.1016/j.ijbiomac.2013.09.025. Epub 2013 Sep 26.
Chitosan biofilms, prepared by casting method at various percentage of plasticizer (PEG and glycerol), were evaluated for their biological, structural and thermal properties. The addition of PEG at 30% (w/w) and glycerol at 10% (w/w) to chitosan has increased the antioxidant activity of biofilm with the percentages of 22 and 26%, respectively. The increase of ferric reducing power was noted for the mixtures of chitosan-PEG (70-30) and chitosan-GLY (75-25). Additionally, the antibacterial properties of several biofilms were tested against E. coli and S. aureus. Biofilms with 70-30 and 90-10 blends ratio of chitosan-PEG and chitosan-GLY showed the best inhibitory effect against E. coli and S. aureus with 12 and 27%, respectively. All biofilms were degraded in compost in liquid and the addition of plasticizer PEG to chitosan increased his biodegradability with a value of BOD5 about 2.33 O2/mg CO. FT-IR spectra showed that the addition of plasticizer promoted the interactions through hydrogen bonding as reflected on the shifting of main peaks but there is no effect on biodegradation.
壳聚糖生物膜通过浇铸法在不同增塑剂（PEG 和甘油）的百分比下制备，评估了其生物、结构和热性能。PEG 添加量为 30%（w/w）和甘油添加量为 10%（w/w）分别提高了生物膜的抗氧化活性，分别为 22%和 26%。还注意到壳聚糖-PEG（70-30）和壳聚糖-GLY（75-25）混合物的铁还原力增加。此外，还测试了几种生物膜对大肠杆菌和金黄色葡萄球菌的抗菌性能。壳聚糖-PEG 和壳聚糖-GLY 的 70-30 和 90-10 混合比例的生物膜对大肠杆菌和金黄色葡萄球菌的抑制效果最好，分别为 12%和 27%。所有生物膜在液体堆肥中降解，添加 PEG 增塑剂可提高壳聚糖的生物降解性，BOD5 值约为 2.33 O2/mg CO。FT-IR 光谱表明，增塑剂的添加通过氢键促进了相互作用，反映在主要峰的移动上，但对生物降解没有影响。
