紫玉盘（番荔枝科）水提物对链脲佐菌素诱导的糖尿病大鼠的降血糖和抗氧化作用。
Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats.
机构信息
Department of Animal Biology and Physiology, Laboratory of Animal Physiology, University of Yaounde I, PO Box 812, Cameroon.
Department of Pharmacy, Université des Montagnes (UdM), PO Box 208, Bangangté, Cameroon.
出版信息
J Ethnopharmacol. 2014 Feb 3;151(2):784-90. doi: 10.1016/j.jep.2013.09.021. Epub 2013 Sep 25.
ETHNOPHARMACOLOGICAL RELEVANCE
The leaves of Annona muricata are used in Cameroon to manage diabetes and its complications. The aim of this study was to evaluate the antidiabetic, antioxidant activities and the potential toxicity of aqueous extract of Annona muricata in streptozotocin-induced diabetic rats.
MATERIAL AND METHODS
Oral administration of Annona muricata aqueous extract (100mg/kg or 200mg/kg) was studied in normal and streptozotocin-induced diabetic rats. In long term treatment, 2 weeks after streptozotocin-induced diabetic rats, animals received plant extract during 28 consecutive days. For a protective effect, extract was administered 3 days prior to streptozotocin exposure and animals were observed 2 weeks without treatment.
RESULTS
The plant extract was not effective in normal rats. In diabetic rats, single administration of the extract significantly reduced blood glucose levels by 75% and 58.22% respectively at the dose of 100mg/kg and 200mg/kg as compared to the initial value. Treatment of normal rats 3 days prior to diabetes induction showed that, Annona muricata extract has no effect within 72h following STZ injection. However, after 14 days post-treatment, the extract at the dose of 100mg/kg significantly reduced blood glucose levels as compared with initial value and diabetic control rats. Immunohistochemical staining of pancreatic β-cells of diabetic rats treated with the dose of 100mg/kg expressed strong staining for β-cell compared to diabetic control. In a long-term study daily administration of Annona muricata aqueous extract for 28 days to diabetic rats, reduced blood glucose levels, serum creatinine, MDA, AST, ALT activity, and nitrite levels LDL-cholesterol. Total cholesterol, triglycerides, SOD, and CAT activity contents were restored.
CONCLUSION
These different results show that the antidiabetic activity of Annona muricata aqueous extract can be explained by its hypolipidaemic effect, its antioxidant and protective action on pancreatic β-cells, which in turn improve glucose metabolism.
民族药理学相关性
在喀麦隆，番荔枝的叶子被用于治疗糖尿病及其并发症。本研究旨在评估番荔枝水提物的抗糖尿病、抗氧化活性和潜在毒性，以研究其对链脲佐菌素诱导的糖尿病大鼠的作用。
材料和方法
研究了番荔枝水提物（100mg/kg 或 200mg/kg）在正常和链脲佐菌素诱导的糖尿病大鼠中的口服给药。在长期治疗中，在链脲佐菌素诱导的糖尿病大鼠 2 周后，动物在 28 天内连续接受植物提取物治疗。为了进行保护作用，提取物在暴露于链脲佐菌素前 3 天给予，并且在没有治疗的情况下观察 2 周。
结果
该植物提取物在正常大鼠中无效。在糖尿病大鼠中，提取物在 100mg/kg 和 200mg/kg 剂量下分别使血糖水平降低了 75%和 58.22%，与初始值相比。在糖尿病诱导前 3 天给予正常大鼠治疗，表明番荔枝提取物在 STZ 注射后 72 小时内没有影响。然而，在治疗 14 天后，与初始值和糖尿病对照大鼠相比，100mg/kg 剂量的提取物显著降低了血糖水平。用 100mg/kg 剂量治疗的糖尿病大鼠的胰腺β细胞免疫组织化学染色显示，β细胞的染色比糖尿病对照大鼠强。在长期研究中，糖尿病大鼠每天给予番荔枝水提物 28 天，可降低血糖水平、血清肌酐、MDA、AST、ALT 活性和硝酸盐水平，降低 LDL-胆固醇。总胆固醇、甘油三酯、SOD 和 CAT 活性含量得到恢复。
结论
这些不同的结果表明，番荔枝水提物的抗糖尿病活性可以通过其降血脂作用、抗氧化和对胰腺β细胞的保护作用来解释，这反过来又改善了葡萄糖代谢。
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