Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamilnadu, India.
Department of Pharmacology, Nandha College of Pharmacy and Research Institute, Erode 638052, India.
J Ethnopharmacol. 2014 Feb 12;151(3):1175-1183. doi: 10.1016/j.jep.2013.12.032. Epub 2013 Dec 27.
Swertia corymbosa locally called as Shirattakuchi have a long history of use in Ayurveda herbal preparations in Indian traditional system of medicine. It has been used in folklore medicine for the treatment of diabetes.
The present study aimed to investigate the effect of the methanolic extract of Swertia corymbosa (SC) in diabetic and to analyze its chemical composition by HPLC-ESI/MS that may correlate with their pharmacological activities.
The in vitro anti-diabetic activity of the extracts was measured by using α-glucosidase and α-amylase enzyme inhibitory activity. The methanolic extract of Swertia corymbosa were administered orally (125, 250 and 500 mg/kg, for 28 days) to streptozotocin-induced diabetic rats. Hypoglycemic effects, oral glucose tolerance test, change in body weight and lipid profile, biochemical analysis and histopathological examination were assessed. High-performance liquid chromatography-electrospray ionization/mass spectrometry (HPLC-ESI/MS) method was also developed to analyze the chemical composition.
In vitro anti-dabetic study, the methanol extract of SC is found to be a potent inhibitor of α-glucosidase and α-amylase activity. Oral administration of SC and standard drug for 28 days caused a significant decrease in the concentrations of blood glucose level, total cholesterol (TC), serum triglycerides (TGs), low-density lipoprotein-cholesterol (LDL-C), malondialdehyde (MDA) and significant increase in the concentrations of high density lipoprotein-cholesterol (HDL-C), serum insulin and body weight. Furthermore, activities of antioxidative enzymes, including SOD, GPx, GSH and CAT were enhanced dosed dependently with SC. Histopathological studies of the pancreas showed the regeneration of the β-cells by extract which were earlier necrosed by streptozotocin. Ten major compounds such as loganic acid (1), swertiamarin (2), sweroside (3), gentiopicroside (4), isovitexin (5), amoroswertin (6), amarogentin (7), gentiacaulein (8), decussatin (9) and swertianin (10) were analyzed by HPLC-ESI/MS system.
These results demonstrate that SC aerial parts of methanolic extract is an effective anti-diabetic and antioxidant activities which provides the scientific proof for the folklore medicine.
在印度传统医学体系的阿育吠陀草药制剂中，川西獐牙菜（Swertia corymbosa）局部称为 Shirattakuchi，已有悠久的使用历史。它在民间医学中一直被用于治疗糖尿病。
本研究旨在探讨川西獐牙菜甲醇提取物（SC）对糖尿病的影响，并通过高效液相色谱-电喷雾/质谱联用（HPLC-ESI/MS）分析其化学成分，这些成分可能与其药理活性相关。
采用α-葡萄糖苷酶和α-淀粉酶酶抑制活性测定提取物的体外抗糖尿病活性。对链脲佐菌素诱导的糖尿病大鼠给予川西獐牙菜甲醇提取物（125、250 和 500 mg/kg，连续 28 天）进行口服给药。评估降血糖作用、口服葡萄糖耐量试验、体重和血脂谱变化、生化分析和组织病理学检查。还开发了高效液相色谱-电喷雾/质谱（HPLC-ESI/MS）法来分析化学成分。
体外抗糖尿病研究表明，SC 甲醇提取物是一种有效的α-葡萄糖苷酶和α-淀粉酶抑制剂。连续 28 天给予 SC 和标准药物可显著降低血糖水平、总胆固醇（TC）、血清甘油三酯（TGs）、低密度脂蛋白胆固醇（LDL-C）、丙二醛（MDA）的浓度，显著增加高密度脂蛋白胆固醇（HDL-C）、血清胰岛素和体重的浓度。此外，抗氧化酶（包括 SOD、GPx、GSH 和 CAT）的活性随着 SC 的剂量依赖性增强而增强。胰腺的组织病理学研究表明，提取物使先前因链脲佐菌素而坏死的β细胞再生。通过 HPLC-ESI/MS 系统分析了 10 种主要化合物，如 loganic 酸（1）、獐牙菜苦苷（2）、獐牙菜苷（3）、龙胆苦苷（4）、荭草苷（5）、麦角甾苷（6）、amaroswertin（7）、amarogentin（8）、decussatin（9）和 swertianin（10）。
这些结果表明，川西獐牙菜甲醇提取物具有有效的抗糖尿病和抗氧化活性，为民间医学提供了科学依据。
