Department of Environmental Technologies, Faculty of Marine and Environmental Sciences (CASEM), University of Cádiz, Pol, Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain.
Bioresour Technol. 2013 Nov;148:443-52. doi: 10.1016/j.biortech.2013.09.002. Epub 2013 Sep 11.
Microbial population dynamics and anaerobic digestion (AD) process to eight different hydraulic retention times (HRTs) (from 25d to 3.5d) in two-phase dry-thermophilic AD from sulphate-containing solid waste were investigated. Maximum values of gas production (1.9 ± 0.2 l H2/l/d; 5.4 ± 0.3 l CH4/l/d and 82 ± 9 ml H2S/l/d) and microbial activities were obtained at 4.5d HRT; where basically comprised hydrolysis step in the first phase (HRT=1.5d) and acidogenic step finished in the second phase as well as acetogenic-methanogenic steps (HRT=3d). In the first phase, hydrolytic-acidogenic bacteria (HABs) was the main group (44-77%) and Archaea, acetogens and sulphate-reducing bacteria (SRBs) contents were not significant; in the second phase (except to 2d HRT), microbial population was able to adapt to change in substrate and HRTs to ensure the proper functioning of the system and both acetogens and Archaea were dominated over SRBs. Decreasing HRT resulted in an increase in microbial activities.
研究了含硫酸盐固体废弃物两相干热厌氧消化（AD）在 8 个不同水力停留时间（HRT）（从 25d 到 3.5d）下的微生物种群动态和 AD 过程。在 4.5d 的 HRT 下，获得了最大的气体产量（1.9±0.2 l H2/l/d；5.4±0.3 l CH4/l/d 和 82±9 ml H2S/l/d）和微生物活性；其中基本包括第一阶段的水解步骤（HRT=1.5d）和第二阶段的产酸步骤以及产酸-产甲烷步骤（HRT=3d）。在第一阶段，水解-产酸菌（HABs）是主要菌群（44-77%），古菌、产乙酸菌和硫酸盐还原菌（SRBs）的含量不显著；在第二阶段（除了 2d HRT），微生物种群能够适应基质和 HRT 的变化，以确保系统的正常运行，产乙酸菌和古菌都超过了 SRBs。HRT 的缩短导致微生物活性的增加。
