1] Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA. [2] St. Laurent Institute, Cambridge, Massachusetts, USA.
Nat Struct Mol Biol. 2013 Nov;20(11):1333-9. doi: 10.1038/nsmb.2675. Epub 2013 Sep 29.
The accurate and thorough genome-wide detection of adenosine-to-inosine editing, a biologically indispensable process, has proven challenging. Here, we present a discovery pipeline in adult Drosophila, with 3,581 high-confidence editing sites identified with an estimated accuracy of 87%. The target genes and specific sites highlight global biological properties and functions of RNA editing, including hitherto-unknown editing in well-characterized classes of noncoding RNAs and 645 sites that cause amino acid substitutions, usually at conserved positions. The spectrum of functions that these gene targets encompass suggests that editing participates in a diverse set of cellular processes. Editing sites in Drosophila exhibit sequence-motif preferences and tend to be concentrated within a small subset of total RNAs. Finally, editing regulates expression levels of target mRNAs and strongly correlates with alternative splicing.
准确而全面地检测腺苷到肌苷的编辑（一种必不可少的生物学过程）一直具有挑战性。在这里，我们在成年果蝇中提出了一个发现管道，其中鉴定了 3581 个高置信度的编辑位点，估计准确率为 87%。靶基因和特定的位点突出了 RNA 编辑的全局生物学特性和功能，包括在已充分研究的非编码 RNA 类别中以前未知的编辑，以及 645 个导致氨基酸替换的位点，通常在保守位置。这些基因靶标所涵盖的功能范围表明，编辑参与了一系列不同的细胞过程。果蝇中的编辑位点表现出序列基序偏好，并且往往集中在总 RNA 的一小部分内。最后，编辑调节靶 mRNA 的表达水平，并与选择性剪接强烈相关。
