• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新型牛痘病毒启动子有望成为未来疫苗的潜在候选物。

New vaccinia virus promoter as a potential candidate for future vaccines.

机构信息

Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

Biocomputing Unit, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

出版信息

J Gen Virol. 2013 Dec;94(Pt 12):2771-2776. doi: 10.1099/vir.0.057299-0. Epub 2013 Sep 28.

DOI:10.1099/vir.0.057299-0
PMID:24077296
Abstract

Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.

摘要

在这里，我们描述了一种新型晚期早期优化（LEO）痘苗病毒（VACV）启动子的设计和强度，该启动子在改良安卡拉痘苗病毒感染期间作为 GFP 表达的转录调节剂。与已描述的合成 VACV 启动子（pS）相比，LEO 在感染后第一个小时内诱导 GFP 表达水平显著提高，这与体内检测到的 GFP 特异性 CD8 T 细胞反应增强相关，表明其在未来疫苗中的潜在用途。

相似文献

1
New vaccinia virus promoter as a potential candidate for future vaccines.新型牛痘病毒启动子有望成为未来疫苗的潜在候选物。
J Gen Virol. 2013 Dec;94(Pt 12):2771-2776. doi: 10.1099/vir.0.057299-0. Epub 2013 Sep 28.
2
Immediate-early expression of a recombinant antigen by modified vaccinia virus ankara breaks the immunodominance of strong vector-specific B8R antigen in acute and memory CD8 T-cell responses.改良安卡拉痘苗病毒表达的重组抗原可打破急性和记忆 CD8 T 细胞反应中强载体特异性 B8R 抗原的免疫优势。
J Virol. 2010 Sep;84(17):8743-52. doi: 10.1128/JVI.00604-10. Epub 2010 Jun 10.
3
Myristoylation increases the CD8+T-cell response to a GFP prototype antigen delivered by modified vaccinia virus Ankara.豆蔻酰化增强了CD8 + T细胞对由改良安卡拉痘苗病毒递送的绿色荧光蛋白原型抗原的反应。
J Gen Virol. 2016 Apr;97(4):934-940. doi: 10.1099/jgv.0.000425. Epub 2016 Feb 10.
4
A novel naturally occurring tandem promoter in modified vaccinia virus ankara drives very early gene expression and potent immune responses.一种新型天然串联启动子在改良安卡拉痘苗病毒中驱动极早期基因表达和有效的免疫应答。
PLoS One. 2013 Aug 12;8(8):e73511. doi: 10.1371/journal.pone.0073511. eCollection 2013.
5
Enhancement of CD8(+) T-cell memory by removal of a vaccinia virus nuclear factor-κB inhibitor.通过去除痘苗病毒核因子-κB抑制剂增强CD8(+) T细胞记忆
Immunology. 2015 May;145(1):34-49. doi: 10.1111/imm.12422.
6
Deletion of gene A41L enhances vaccinia virus immunogenicity and vaccine efficacy.基因A41L的缺失增强了痘苗病毒的免疫原性和疫苗效力。
J Gen Virol. 2006 Jan;87(Pt 1):29-38. doi: 10.1099/vir.0.81417-0.
7
Maintenance of CD8(+) T-cell memory following infection with recombinant sindbis and vaccinia viruses.感染重组辛德毕斯病毒和痘苗病毒后CD8(+) T细胞记忆的维持。
Virology. 2000 Apr 25;270(1):54-64. doi: 10.1006/viro.2000.0255.
8
Characterizing the effects of insertion of a 5.2 kb region of a VACV genome, which contains known immune evasion genes, on MVA immunogenicity.研究插入含有已知免疫逃避基因的 VACV 基因组 5.2kb 区域对 MVA 免疫原性的影响。
Virus Res. 2018 Feb 15;246:55-64. doi: 10.1016/j.virusres.2018.01.004. Epub 2018 Jan 16.
9
Robust intrapulmonary CD8 T cell responses and protection with an attenuated N1L deleted vaccinia virus.用减毒的缺失N1L痘苗病毒诱导强大的肺内CD8 T细胞应答及产生保护作用。
PLoS One. 2008 Oct 2;3(10):e3323. doi: 10.1371/journal.pone.0003323.
10
Modification of promoter spacer length in vaccinia virus as a strategy to control the antigen expression.痘苗病毒启动子间隔长度的修饰作为控制抗原表达的一种策略。
J Gen Virol. 2015 Aug;96(8):2360-2371. doi: 10.1099/vir.0.000183. Epub 2015 May 13.

引用本文的文献

1
Advancing ORFV-Based Therapeutics to the Clinical Stage.将基于羊口疮病毒的疗法推进到临床阶段。
Rev Med Virol. 2025 May;35(3):e70038. doi: 10.1002/rmv.70038.
2
Development of a dual vaccine against East Coast fever and lumpy skin disease.开发一种针对东非锥虫病和块状皮肤病的双价疫苗。
Front Immunol. 2023 Mar 30;14:1143034. doi: 10.3389/fimmu.2023.1143034. eCollection 2023.
3
Lumpy Skin Disease-An Emerging Cattle Disease in Europe and Asia.结节性皮肤病——欧洲和亚洲一种新出现的牛病
Vaccines (Basel). 2023 Mar 2;11(3):578. doi: 10.3390/vaccines11030578.
4
A Single Dose of an MVA Vaccine Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Protein Neutralizes Variants of Concern and Protects Mice From a Lethal SARS-CoV-2 Infection.一种表达融合前稳定的 SARS-CoV-2 刺突蛋白的 MVA 疫苗单次接种即可中和关注变体，并保护小鼠免受致死性 SARS-CoV-2 感染。
Front Immunol. 2022 Jan 27;12:824728. doi: 10.3389/fimmu.2021.824728. eCollection 2021.
5
Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector.用作疫苗载体的重组牛结节性皮肤病病毒（LSDV）生长与构建的进展
Vaccines (Basel). 2021 Oct 4;9(10):1131. doi: 10.3390/vaccines9101131.
6
Construction and immune protection evaluation of recombinant virus expressing Newcastle disease virus F protein by the largest intergenic region of fowlpox virus NX10.利用禽痘病毒 NX10 的最大基因间区构建并评价表达新城疫病毒 F 蛋白的重组病毒及其免疫保护效果。
Virus Genes. 2020 Dec;56(6):734-748. doi: 10.1007/s11262-020-01799-5. Epub 2020 Oct 3.
7
Viral Delivery of CAR Targets to Solid Tumors Enables Effective Cell Therapy.将嵌合抗原受体（CAR）靶点通过病毒递送至实体瘤可实现有效的细胞治疗。
Mol Ther Oncolytics. 2020 Apr 7;17:232-240. doi: 10.1016/j.omto.2020.03.018. eCollection 2020 Jun 26.
8
An MVA Vector Expressing HIV-1 Envelope under the Control of a Potent Vaccinia Virus Promoter as a Promising Strategy in HIV/AIDS Vaccine Design.一种在强效痘苗病毒启动子控制下表达HIV-1包膜的改良痘苗病毒安卡拉载体，作为HIV/AIDS疫苗设计中一种有前景的策略。
Vaccines (Basel). 2019 Dec 6;7(4):208. doi: 10.3390/vaccines7040208.
9
Genes A27L and F13L as Genetic Markers for the Isolation of Recombinant Vaccinia Virus.A27L 和 F13L 基因作为分离重组痘苗病毒的遗传标记。
Sci Rep. 2019 Oct 30;9(1):15684. doi: 10.1038/s41598-019-52053-4.
10
Genomic Characterization of Strain D1701-V () and Development of Novel Sites for Multiple Transgene Expression.D1701-V()株的基因组特征与多个转基因表达新位点的开发
Viruses. 2019 Jan 30;11(2):127. doi: 10.3390/v11020127.