Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143 St., 90-236 Łódź, Poland.
Environ Toxicol Pharmacol. 2013 Nov;36(3):979-88. doi: 10.1016/j.etap.2013.08.014. Epub 2013 Sep 8.
In this study, we have assessed apoptotic effect of 1,2,4-trichlorobenzene, hexachlorobenzene, lindane and dieldrin on human peripheral blood lymphocytes. We observed an increase in ROS formation and a decrease in mitochondrial transmembrane potential in the cells incubated with low concentrations of all compounds studied, in particular lindane and dieldrin. ROS formation and changes in mitochondrial transmembrane potential may have influenced caspase-3 activation, a crucial enzyme in the apoptotic process. Moreover, chlorobenzenes, and in particular lindane and dieldrin changed cells' membrane permeability and induced phosphatidylserine translocation, which confirmed that they are capable of inducing apoptosis in human lymphocytes. Apoptotic changes in human lymphocytes provoked by biologically relevant concentrations of these substances suggest that they may disturb function of immunological system especially among people occupationally exposed to their action.
在这项研究中，我们评估了 1,2,4-三氯苯、六氯苯、林丹和狄氏剂对人外周血淋巴细胞的凋亡作用。我们观察到，在用所有研究化合物的低浓度孵育的细胞中，ROS 的形成增加，线粒体跨膜电位降低，尤其是林丹和狄氏剂。ROS 的形成和线粒体跨膜电位的变化可能影响了细胞凋亡过程中的关键酶 caspase-3 的激活。此外，氯苯，特别是林丹和狄氏剂改变了细胞的膜通透性，并诱导了磷脂酰丝氨酸易位，这证实了它们能够诱导人淋巴细胞凋亡。这些物质在生物学相关浓度下引起的人淋巴细胞凋亡变化表明，它们可能会干扰免疫系统的功能，尤其是在那些职业接触这些物质的人群中。
