Pakpour Fatemeh, Najafpour Ghasem, Tabatabaei Meisam, Tohidfar Masoud, Younesi Habiboallah
Bioprocess Biosyst Eng. 2014 May;37(5):923-30. doi: 10.1007/s00449-013-1064-6.
Biohydrogen production through water–gas shift (WGS) reaction by a biocatalyst was conducted in batch fermentation. The isolated photosynthetic bacterium Rhodopseudomonas palustris PT was able to utilize carbon monoxide and simultaneously produce hydrogen. Light exposure was provided as an indispensable requirement for the first stage of bacterial growth, but throughout the hydrogen production stage, the energy requirement was met through the WGS reaction. At ambient pressure and temperature, the effect of various sodium acetate concentrations in presence of CO-rich syngas on cell growth, carbon monoxide consumption, and biohydrogen production was also investigated. Maximal efficiency of hydrogen production in response to carbon monoxide consumption was recorded at 86 % and the highest concentration of hydrogen at 33.5 mmol/l was achieved with sodium acetate concentration of 1.5 g/l. The obtained results proved that the local isolate; R. palustris PT, was able to utilize CO-rich syngas and generate biohydrogen via WGS reaction.
通过生物催化剂的水煤气变换（WGS）反应进行生物制氢是在分批发酵中进行的。分离出的光合细菌沼泽红假单胞菌PT能够利用一氧化碳并同时产生氢气。光照是细菌生长第一阶段必不可少的条件，但在整个产氢阶段，能量需求通过WGS反应来满足。在常压和常温下，还研究了在富含CO的合成气存在下不同乙酸钠浓度对细胞生长、一氧化碳消耗和生物制氢的影响。一氧化碳消耗的最大产氢效率记录为86%，乙酸钠浓度为1.5 g/l时，氢气最高浓度达到33.5 mmol/l。所得结果证明，本地分离株沼泽红假单胞菌PT能够利用富含CO的合成气并通过WGS反应产生生物氢。
