Hanisch U K, Rothe T, Webster H H, Härtig W, Biesold D
Paul Flechsig Institute for Brain Research, Department of Neurochemistry, Karl Marx University, Leipzig (G.D.R.).
J Neurosci Methods. 1990 Jan;31(1):53-8. doi: 10.1016/0165-0270(90)90009-5.
A method for the rapid dissection of circumscribed areas of rat cortex is described. The technique does not depend on skull-derived landmarks but uses for stereotaxic orientation the cross-point of the interhemispheric gap with the caudal margin of the cortex. An application of this dissection method to the biochemical analysis of cholinergic markers within the hindlimb representation of the primary somatosensory cortex revealed that both the activity of the enzyme choline acetyltransferase as well as the binding of [3H] quinuclidinyl benzilate to muscarinic cholinergic receptors do not seem to be affected drastically three days after unilateral transection of the sciatic nerve. The only significant effect detected was a slight decrease in the activity of the choline acetyltransferase within the hindlimb representation of the primary somatosensory cortex contralateral to the transected sciatic nerve. In the primary visual cortex, the cholinergic markers investigated did not show significant alterations after sciatic nerve injury.
本文描述了一种快速解剖大鼠皮质特定区域的方法。该技术不依赖于颅骨来源的标志，而是利用半球间间隙与皮质尾缘的交叉点进行立体定位。将这种解剖方法应用于初级体感皮质后肢代表区内胆碱能标志物的生化分析，结果显示，在坐骨神经单侧横断三天后，胆碱乙酰转移酶的活性以及[3H]喹核醇基苯甲酸酯与毒蕈碱胆碱能受体的结合似乎均未受到显著影响。检测到的唯一显著效应是，在与横断坐骨神经对侧的初级体感皮质后肢代表区内，胆碱乙酰转移酶的活性略有下降。在初级视觉皮质中，坐骨神经损伤后所研究的胆碱能标志物未显示出显著变化。
