高效液相色谱-质谱联用（HPLC-MS）和气相色谱-质谱联用（GC-MS）分析结合正交偏最小二乘法以鉴定姜黄（Curcuma longa L.）中的细胞毒性成分。
HPLC-MS and GC-MS analyses combined with orthogonal partial least squares to identify cytotoxic constituents from turmeric (Curcuma longa L.).
作者信息
Jiang Jianlan, Zhang Huan, Li Zidan, Zhang Xiaohang, Su Xin, Li Yan, Qiao Bin, Yuan Yingjin
机构信息
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin Key Laboratory of Biological and Pharmaceutical Engineering, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
出版信息
Nat Prod Commun. 2013 Aug;8(8):1129-34.
We investigated the fingerprints of 48 batches of turmeric total extracts (TTE) by HPLC-MS-MS and GC-MS analyses and 43 characteristic peaks (22 constituents from HPLC-MS-MS; 21 from GC-MS) were analyzed qualitatively and quantitatively. An MTT {3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide} assay was implemented to measure the cytotoxicity of the TTE against HeLa cells. Then we utilized orthogonal partial least squares analysis, which correlated the chemical composition of the TTE to its cytotoxic activity, to identify potential cytotoxic constituents from turmeric. The result showed that 19 constituents contributed significantly to the cytotoxicity. The obtained result was verified by canonical correlation analysis. Comparison with previous reports also indicated some interaction between the curcuminoids and sesquiterpenoids in turmeric.
我们通过高效液相色谱-串联质谱（HPLC-MS-MS）和气相色谱-质谱（GC-MS）分析研究了48批次姜黄总提取物（TTE）的指纹图谱，并对43个特征峰（HPLC-MS-MS鉴定出22种成分；GC-MS鉴定出21种成分）进行了定性和定量分析。采用MTT {3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐} 法检测TTE对人宫颈癌HeLa细胞的细胞毒性。然后我们利用正交偏最小二乘法分析，将TTE的化学成分与其细胞毒性活性相关联，以鉴定姜黄中潜在的细胞毒性成分。结果表明，19种成分对细胞毒性有显著贡献。所得结果通过典型相关分析得到验证。与先前报道的比较还表明姜黄中的姜黄素类化合物和倍半萜类化合物之间存在一些相互作用。
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