Hamia Chahrazed, Gourine Nadhir, Boussoussa Hadjer, Saidi Mokhtar, Gaydou Emile M, Yousfi Mohamed
Laboratoire des Sciences Fondamentales a l'Université Amar TELIDJI de Laghouat, (03000), Algeria.
Nat Prod Commun. 2013 Aug;8(8):1171-4.
The essential oil obtained by hydrodistillation of the flowers of Rhanterium adpressum Coss. & Durieu was analyzed using GC and GC-MS. The essential oil was very rich in monoterpene compounds. The major components identified were the monoterpene hydrocarbons: camphene (21.8%), myrcene (19.3%) and alpha-pinene (17.4%). Other compounds, including limonene, beta-pinene and terpinol-4-ol, were present in low content (4-6%). The composition of the fatty acids in the lipid extract obtained from the flowers was also investigated by GC and GC-MS. The main fatty acids identified were palmitic (47.4%), oleic (12.9%) and stearic acids (10.6%). The total phenolic contents and the antioxidant activities were also evaluated for both extracts. The total phenolic contents were determined using the Folin-Ciocalteu reagent and the antioxidant activities were measured using three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, FRAP (ferric reducing antioxidant potential) and a molybdenum assay. As a result of these tests, the lipid extract exhibited the highest antioxidant activities in comparison with the essential oil extract.
采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）技术，对从贴生旱霸王（Rhanterium adpressum Coss. & Durieu）花朵中通过水蒸馏法获得的精油进行了分析。该精油富含单萜类化合物。鉴定出的主要成分是单萜烃类：莰烯（21.8%）、月桂烯（19.3%）和α-蒎烯（17.4%）。其他化合物，包括柠檬烯、β-蒎烯和4-松油醇，含量较低（4-6%）。还通过GC和GC-MS对从花朵中获得的脂质提取物中的脂肪酸组成进行了研究。鉴定出的主要脂肪酸为棕榈酸（47.4%）、油酸（12.9%）和硬脂酸（10.6%）。对两种提取物的总酚含量和抗氧化活性也进行了评估。使用福林-酚试剂测定总酚含量，并使用三种不同的测定方法测量抗氧化活性：1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、铁还原抗氧化能力（FRAP）和钼测定法。这些测试的结果表明，与精油提取物相比，脂质提取物表现出最高的抗氧化活性。
